Define DNA ligase. DNA ligase synonyms, DNA ligase pronunciation, DNA ligase translation, English dictionary definition of DNA ligase. DNA A. adenine T. thymine C. cytosine G. guanine n. A nucleic acid that carries the genetic information in cells and so
The genomic DNA ofT. mongolicuswas extracted from the leaves using a modified CTAB method [34]. Quality of the isolated DNA was assessed using NanoDrop-2000 (Thermo Fisher Scientific, Wilmington, DE, USA) and Qubit 3.0 fluorometer (Life Technologies). The genomic DNA was sheared to an average...
Intrinsic apoptosis of mitochondrial pathway: DNA damage, growth factor deficiency, ROS and other endogenous stress cause the Bad/Bak proteins (respectively pro-apoptotic members of the Bcl-2 family) to form a complex, which is inserted into the mitochondrial outer membrane, changes the permeability ...
A 35-base Target DNA complementary to the Capture and the Reporter probes was used as Template in the ligation process, with the ligation between the Reporter and Capture probes mediated by T4 ligase. Iron oxide magnetic nanoparticles functionalized with carboxylic groups on their surface synthesized...
and DNA repair. Their multifaceted role in steering the destiny of stem cells is evident, as they wield influence over self-renewal, quiescence, and lineage-specific differentiation in both embryonic and adult stem cells. This review delves into the structural and regulatory intricacies of FOXO tran...
associated proteins. Among these, we focused on C13ORF7/RNF219, an uncharacterised potential E3 ubiquitin ligase that we called OBI1 (ORC ubiquitin-ligase-1). The properties of OBI1 suggest that it could be a replication origin selector essential for DNA replication origin activation during S...
如图6A所示,单体/ mRNA复合物从DNA池中合成,其中单体BC-FG或CD-FG环的编码区域是随机的。反转录后,将单体/cDNA复合物与生物素化的重组OPTN片段孵育,并用链霉亲和素包被磁珠处理,从而选择对OPTN具有亲和力的复合物。将扩增的单体dna重新引入TRAP系统,以产生单体文库。他们针对两个不同的n端OPTN区域(26 - 196aa...
TD-60 (also known as RCC2) is a highly conserved protein that structurally resembles the Ran guanine exchange factor (GEF) RCC1, but has not previously been shown to have GEF activity. TD-60 has a typical chromosomal passenger complex (CPC) distribution
1 μ1 of 10 x T4 DNA ligase buffer, 20 ng of pYLgRNA-OsU6a or pYLgRNA-OsU3 vector, 1 μ1 of the target sequence adaptor, and 35 U of T4 DNA ligase (Takara, Dalian, China). The target sequence adaptors TMS5a, TMS5c, TMS5e, TMS5g and TMS5i, respectively, were ligated ...
The conversion of the prion protein (PrPC) into prions plays a key role in transmissible spongiform encephalopathies. Despite the importance for pathogenesis, the mechanism of prion formation has escaped detailed characterization due to the insoluble nat