How are chromatin, chromosomes, and DNA related? What is the basic structure of DNA? How is the structure of DNA a common language? How does the structure of DNA encode genetic information? How does DNA ligase work? How does water affect DNA structure?
How does DNA microarray label DNA pieces? How does DNA ligase work? How does protein synthesis enable DNA to control the cell? How does DNA microarray technology work? How is DNA replication used in biotechnology? How did people design primers without known DNA sequences?
Finally, from the DNA of one cell, we can clone an animal, a plant or perhaps even a human being. But what is DNA? Where is it found? What makes it so special? How does it work? In this article, we will look deep into the structure of DNA and explain how it makes itself and...
New findings from the Reverter and Torres‐Rosell laboratories in The EMBO Journal demonstrate that vicinity of preferentially ssDNA activates the SUMO E3 ligase Nse2 when in complex with SMC5‐6.doi:10.15252/embj.201899705Andrea PichlerJohn Wiley & Sons, Ltd.The EMBO Journal...
DNA polymerases were named for their function of catalysing DNA replication, a process that is necessary for growth and propagation of life. DNA involving Watson–Crick base-pairing can be synthesized with high fidelity, the structural and mechanistic or
The substrate for ligase is a bridge structure formed by hybridization of a third oligonucleotide to the oligonucleotide ends of the assay probe pair. This structure forms preferentially when the assay probes are brought into proximity by binding to the target protein. ...
Aerobic glycolysis and DNA methylation not only work together to regulate tumor cells directly, but also may influence the development of tumors by regulating the function of the immune system. Aerobic glycolysis is considered to be a metabolic hallmark of activated T cells. Pearce EL et al. sugg...
PCR products are then ligated with T4 DNA ligase. This effectively moves the restriction enzyme sites away from the ends of the fragments and allows efficient digestion. Back to top References Kaufman, D.L. and Evans, G.A. (1990) Restriction endonuclease cleavage at the termini of PCR ...
The C-terminal Ring domain of UHRF1 works as an E3 ligase and performs histone H3K23 ubiquitylation, which favours DNMT1 recruitment on hemi-methylated CG. H3K23 ubiquitylation antagonises the auto-inhibitory effect of the DNMT1’s RFTS domain (The Replication Focus Targeting Sequence) (Liu ...
PCR products are then ligated with T4 DNA ligase. This effectively moves the restriction enzyme sites away from the ends of the fragments and allows efficient digestion. Back to top References Kaufman, D.L. and Evans, G.A. (1990) Restriction endonuclease cleavage at the termini of PCR ...