Steven D. LeachMarnie E. HalpernElsevier Inc.Developmental BiologyAkitake CM, Goll MG, Rhee J, Parsons MJ, Leach SD, Halpern ME. 2008. Optimization of the Gal4-UAS system and generation of a versatile collection of UAS-reporters for zebrafish. Dev Biol 319:344....
a A diagram to show different brain regions in the adult fly (top) and the inactivation scheme by the GAL4/UAS system (bottom). Different Gal4s drove the expression of TNT in specific neurons in selected brain regions, including the mushroom body (b), ellipsoid body (c), fan-shaped body...
The mitoQC assay may also be performed inDrosophilato quantify mitophagy specifically in cell types of interest using the GAL4/UAS system. Briefly, we expressed the mitoQC transgene, in dopaminergic neurons using the TH-GAL4 driver38. Since CCCP cannot be administered without affecting the viabili...
By combining the new GCaMP with targeted gene expression via the Gal4FF-UAS system, we successfully imaged the activity of the spinal motor circuit ... A Muto,M Ohkura,T Kotani,... - 《Proceedings of the National Academy of Sciences of the United States of America》 被引量: 221发表: ...
By combining the new GCaMP with targeted gene expression via the Gal4FF-UAS system, we successfully imaged the activity of the spinal motor circuit during spontaneous contractions of zebrafish larvae. Further we report here that heptanol, a gap junction blocker, could alter the spatiotemporal ...
The LexA/LexAop system (Lai and Lee, 2006) is similar to the Gal4/UAS system (Brand and Perrimon, 1993). There are no available LexA drivers specific to the gut, whereas such Gal4 drivers exist, so we searched for LexAs to use for sleep deprivation. After screening through ∼150 ...
To facilitate tissue-specific analysis of Dichaete action, we generated dominant negative (DN) Dichaete proteins and expressed them in specific tissues via the GAL4-UAS system [25]. We produced three types of construct: two dominant negative proteins, either lacking the Dichaete HMG DNA-binding...
In order to assess the role of specific neurons in the modulation of larval photobehavior, we used the GAL4/UAS system [33]. In this approach, specific enhancers or promoters are used to regulate the expression of the yeast transcription factor GAL4. A gene of interest, such as tetanus to...
A GAL4/UAS luciferase system to identify the miRNAs target mRNAs in Drosophila S2 cellsmiRNAsUAS/GAL4 systemS2 cellMicroRNAs (miRNAs) have been shown to regulate gene expression through the sequence-specific base pairing with their target mRNAs. However, our understanding of the biological roles ...
Ito, K., Sass, H., Urban, J.et al.GAL4-responsive UAS-tauas a tool for studying the anatomy and development of theDrosophilacentral nervous system.Cell Tissue Res290, 1–10 (1997). https://doi.org/10.1007/s004410050901 Download citation ...