Quantitative analysis of the GAL4/UAS system in Drosophila oogenesis. Genesis 44, 66-74.Goentoro, L. A., N. Yakoby, J. Goodhouse, T. Schupbach, and S. Y. Shvartsman, 2006 Quantitative analysis of the GAL4/UAS system in Drosophila oogenesis. Genesis 44: 66-74....
We show that this functions in vivo in the widely used Gal4-UAS bipartite expression system in Drosophila melanogaster. By regulating a cell death gene, we demonstrate that only the low background double architecture enables tight regulation of the lethal phenotype in vivo. These improved tools ...
The mitoQC assay may also be performed inDrosophilato quantify mitophagy specifically in cell types of interest using the GAL4/UAS system. Briefly, we expressed the mitoQC transgene, in dopaminergic neurons using the TH-GAL4 driver38. Since CCCP cannot be administered without affecting the viabili...
a A diagram to show different brain regions in the adult fly (top) and the inactivation scheme by the GAL4/UAS system (bottom). Different Gal4s drove the expression of TNT in specific neurons in selected brain regions, including the mushroom body (b), ellipsoid body (c), fan-shaped body...
Besides the clonal analysis, we took advantage of the GAL4-UAS system (Brand and Perrimon 1993) to appose cells with different levels of fz expression as described previously (Adler et al. 1997) and examined how the distribution of Fmi was altered. The patched (ptc)-GAL4 driver was used ...
In order to assess the role of specific neurons in the modulation of larval photobehavior, we used the GAL4/UAS system [33]. In this approach, specific enhancers or promoters are used to regulate the expression of the yeast transcription factor GAL4. A gene of interest, such as tetanus to...
To facilitate tissue-specific analysis of Dichaete action, we generated dominant negative (DN) Dichaete proteins and expressed them in specific tissues via the GAL4-UAS system [25]. We produced three types of construct: two dominant negative proteins, either lacking the Dichaete HMG DNA-binding...
GAL4-responsive UAS- tau as a tool for studying the anatomy and development of the Drosophila central nervous system 1997. GAL4-responsive UAS-tau as a tool for studying the anatomy and development of the Drosophila central nervous system. Cell Tissue Res 290:1-10... S Schneuwly,A Hofbaue...
The reporter gene constructs (GAL4-UAS) and effector constructs (SPL4, SPL4-d3, SPL4-d15) were performed as previously described (Lyu et al.,2020). Briefly, the GAL4-UAS was cloned and inserted into the pGreenII 0800-LUC vector as the reporter. The full-length CDSs of OsSPL4, Os...
In order to understand more about the signalling and to some extent start to unravel the functional roles of these two substances, this study has examined the locations of the transmitters and their receptors in the brain of the fruit fly using immunocytochemistry in combination with Gal4/UAS ...