2011. Tol2-mediated transgenesis, gene trapping, enhancer trapping, and the Gal4-UAS system. Methods Cell Biol, 104, 23-49.Abe, G., Suster, M. L., & Kawakami, K. (2011). Tol2-mediated Transgenesis, gene trapping, enhancer trapping, and the Gal4-UAS System . Methods in cell biology...
a A diagram to show different brain regions in the adult fly (top) and the inactivation scheme by the GAL4/UAS system (bottom). Different Gal4s drove the expression of TNT in specific neurons in selected brain regions, including the mushroom body (b), ellipsoid body (c), fan-shaped body...
The mitoQC assay may also be performed inDrosophilato quantify mitophagy specifically in cell types of interest using the GAL4/UAS system. Briefly, we expressed the mitoQC transgene, in dopaminergic neurons using the TH-GAL4 driver38. Since CCCP cannot be administered without affecting the viabili...
GAL4-responsive UAS- tau as a tool for studying the anatomy and development of the Drosophila central nervous system 1997. GAL4-responsive UAS-tau as a tool for studying the anatomy and development of the Drosophila central nervous system. Cell Tissue Res 290:1-10... S Schneuwly,A Hofbaue...
To facilitate tissue-specific analysis of Dichaete action, we generated dominant negative (DN) Dichaete proteins and expressed them in specific tissues via the GAL4-UAS system [25]. We produced three types of construct: two dominant negative proteins, either lacking the Dichaete HMG DNA-binding...
In order to assess the role of specific neurons in the modulation of larval photobehavior, we used the GAL4/UAS system [33]. In this approach, specific enhancers or promoters are used to regulate the expression of the yeast transcription factor GAL4. A gene of interest, such as tetanus to...
Besides the clonal analysis, we took advantage of the GAL4-UAS system (Brand and Perrimon 1993) to appose cells with different levels of fz expression as described previously (Adler et al. 1997) and examined how the distribution of Fmi was altered. The patched (ptc)-GAL4 driver was used ...
To express these proteins inDrosophila, the GAL4-UAS system was used. The effect of these proteins was monitored in the sexually dimorphic regions of the fly: the foreleg basitarsus, the 5th, 6th and 7th tergites, and the external terminalia. In addition, we analysed the effect ofAnastrep...
The reporter gene constructs (GAL4-UAS) and effector constructs (SPL4, SPL4-d3, SPL4-d15) were performed as previously described (Lyu et al.,2020). Briefly, the GAL4-UAS was cloned and inserted into the pGreenII 0800-LUC vector as the reporter. The full-length CDSs of OsSPL4, Os...
In order to determine the expression pattern ofsick, we generatedsickT2A−GAL4;UAS-mCherry.NLSflies. The GAL4 drives the expression of nuclear mCherry. Third instar larvae exhibit robust expression of the reporter in the CNS with sparse expression in the trachea (Fig.6C). To reveal the pr...