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For a biocatalyst to be effective in an industrial process, it must be subjected to improvement and optimization, and in this respect the directed evolution of enzymes has emerged as a powerful enabling technology. Directed evolution involves repeated rounds of (i) random gene library generation, ...
Although the activities of designed catalysts have been quite low, extensive laboratory evolution has been used to generate efficient enzymes. Structural analysis of these systems has revealed the high degree of precision that will be needed to design catalysts with greater activity. To this end, ...
Over the past few years, there has been a surge in the industrial production of recombinant enzymes from microorganisms due to their catalytic characteristics being highly efficient, selective, and biocompatible. l-asparaginase (l-ASNase) is an enzyme belonging to the class of amidohydrolases that ...
Directed evolution ofPseudomonas aeruginosalipase by the use of combinatorial active site saturation test (CAST) criteria provided a highly enantioselective mutant (Leu162Phe) for kinetic resolution of an axially chiral allene,p-nitrophenyl 4-cyclohexyl-2-methylbuta-2,3-dienoate (E= 111); the high...
Arnold was seeking ways to make improved enzymes, which are proteins that encourage chemical reactions to occur. In 1993, she showed the power of “directed evolution” for doing that. First she created random mutations in DNA that lets cells produce an enzyme. Then she slipped these mutated ...
, enzymes adapted to hot environments show significantly different optimum temperatures than those from organisms adapted to the cold; it is increasingly clear that these activity differences are not due only to differences in stability, but rather reflect a tailoring of enzyme dynamics by evolution....
R. Directed evolution can rapidly improve the activity of chimeric assembly-line enzymes. Proc. Natl Acad. Sci. USA 104, 11951–11956 (2007). CAS PubMed PubMed Central Google Scholar Evans, B. S., Chen, Y., Metcalf, W. W., Zhao, H. & Kelleher, N. L. Directed evolution of the...
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