RNA enzyme-directed therapyAltman, Sidney
Evolution of a designed retro-aldolase leads to complete active site remodeling. Nat. Chem. Biol. 9, 494–498 (2013). Article CAS PubMed PubMed Central Google Scholar Goddard, J. P. & Reymond, J. L. Enzyme assays for high-throughput screening. Curr. Opin. Biotechnol. 15, 314–322 ...
RNA enzyme-directed gene therapy Not Available S Altman - 《Proceedings of the National Academy of Sciences of the United States of America》 被引量: 220发表: 1993年 Prodrugs for Gene-Directed Enzyme-Prodrug Therapy (Suicide Gene Therapy) pThis review focuses on the prodrugs used in suicide ...
Directed evolution experiments are typically carried out using in vitro systems, bacteria, or yeast—even when the goal is to probe or modulate mammalian biology. Performing directed evolution in systems that do not match the intended mammalian environme
There is little relationship between eukaryotic RNA-directed RNA polymerases (RDRs), viral RNA-dependent RNA polymerases (RdRps) and DNA-dependent RNA polymerases, indicating that RDRs evolved as an independent class of enzymes early in evolution. In fungi, plants and several animal systems, RDRs ...
RNA Directed DNA Polymerase In subject area: Pharmacology, Toxicology and Pharmaceutical Science Reverse transcriptase (RT), also known as RNA-dependent DNA polymerase, is a DNA polymerase enzyme that transcribes single-stranded RNA into DNA. From: Encyclopedia of Biological Chemistry (Second ...
Directed evolution of orthogonal aminoacyl-tRNA synthetases (AARSs) enables site-specific installation of noncanonical amino acids (ncAAs) into proteins. Traditional evolution techniques typically produce AARSs with greatly reduced activity and selectivity compared to their wild-type counterparts. We designed...
CSR has applications in the evolution of polymerases with novel and useful properties. By using three cycles of CSR, we obtained variants of Taq DNA polymerase with 11-fold higher thermostability than the wild-type enzyme or with a >130-fold increased resistance to the potent inhibitor heparin....
This review describes and analyses virus adaptation studies performed with members of four RNA virus families that are used for viral oncolysis: reoviruses, paramyxoviruses, enteroviruses, and rhabdoviruses.Keywords: oncolytic viruses, virus selection, virus adaptation, directed viral evol...
To evolve the desired property, an appropriate assay for the property of interest must be chosen. Here, we describe recent advances in the development of in vitro and in vivo diversification methods, as well as high-throughput assays for protein directed evolution. Using recent examples, we ...