Reetz, M. T. Directed Evolution of Selective Enzymes: Catalysts for Organic Chemistry and Biotechnology. (Wiley-VCH Verlag GmbH & Co. KGaA, 2016), https://doi.org/10.1002/9783527655465.Reetz,M.T. (2016) Directed Evolution of Selective Enzymes: Catalysts for Organic Chemistry and Biotechnology....
Directed Evolution of Enzymes for Organic Synthesis Continuing the theme of industrially viable catalysts,Manfred Reetz (Max Planck Institute) presented his latest work on Directed Evolution of Enzymes for organic synthesis,where enzymes can be designed for stability and enantioselectivit... CW Group -...
et al. Evolution of enzyme catalysts caged in biomimetic gel-shell beads. Nat. Chem. 6, 791–796 (2014). In this study, polyelectrolyte shells served as in vitro compartments for screening by flow cytometry. Article CAS PubMed Google Scholar Bessette, P. H., Rice, J. J. & Daugherty,...
Engineered proteins are being widely developed and employed in applications ranging from enzyme catalysts to therapeutic antibodies. Directed evolution, an iterative experimental process composed of mutagenesis and library screening, is a powerful technique for enhancing existing protein activities and ...
The recent discovery of IsPETase, a hydrolytic enzyme that can deconstruct poly(ethylene terephthalate) (PET), has sparked great interest in biocatalytic approaches to recycle plastics. Realization of commercial use will require the development of robust engineered enzymes that meet the demands of indus...
Given the prevalence of radicalrelay reactions in organic synthesis and the diversity of nonheme iron enzymes, we envision that thisdiscovery will stimulate future development of metalloenzyme catalysts for synthetically usefultransformations unexplored by natural evolution.Introducing abiological chemical trans...
Cytochrome P450s: Potential Catalysts for Asymmetric Olefin Epoxidations the use of P450s expressed in microbial hosts, immobilized enzyme systems and directed evolution of individual P450s to provide variants with properties bette... Carlos A. Martinez, Jon D. Stewart - 《Current Organic Chemistry》...
Protein-based hybrid catalysts-design and evolution Artificial metalloenzymes result from the introduction of a catalytically competent non-native metal cofactor within a protein environment. In the present ... Valentin Khler,YM Wilson,C Lo,... - 《Current Opinion in Biotechnology》 被引量: 87发...
What methods exist for high throughput optimization of enzyme activity by directed evolution?Question:What methods exist for high throughput optimization of enzyme activity by directed evolution?EnzymeAn enzyme is a protein and is a biological catalyst. Catalysts speed up chemic...
Currently, enzymes are widely used as catalysts for the production of chiral compounds; however, for further use of enzymes in chiral compound production, improvement of enzymes should be more essential, as well as discovery of novel enzymes and enzyme functions.Kataoka...