We have studied dynamics of an enzyme during catalysis at atomic resolution using nuclear magnetic resonance relaxation methods. During catalytic action of the enzyme cyclophilin A, we detect conformational fluctuations of the active site that occur on a time scale of hundreds of microseconds. The ...
Here we show that the intrinsic plasticity of the protein is a key characteristic of catalysis. The dynamics of the prolyl cis-trans isomerase cyclophilin A (CypA) in its substrate-free state and during catalysis were characterized with NMR relaxation experiments. The characteristic enzyme motions ...
& Kern, D. Enzyme dynamics during catalysis. Science 295, 1520–1523 (2002). 4. Eisenmesser, E. Z. et al. Intrinsic dynamics of an enzyme underlies catalysis. Nature 438, 117–121 (2005). 5. Wolf-Watz, M. et al. Linkage between dynamics and catalysis in a thermophilic-mesophilic ...
摘要: On the base of simple exposition to enzyme and enzyme catalysis, emphatically briefing the dynamics of enzyme catalysis, M - M equation and it' s various of influence factors.关键词: enzyme dynamics of enzyme catalysis M - M equation ...
Crystal structures of three pericyclases, computational simulations of potential energies and molecular dynamics, and site-directed mutagenesis establish the mechanism of this transformation. This work shows how enzymes are able to catalyse concerted pericyclic reactions involving ambimodal transition states....
functions to simulate the domain rotation. The analysis showed that most of the hinge residues undergo small changes at their main chain,φ/ψangles (<15°) while motion of the side chains with variations in χ torsion angles of about 50° was observed. The internal dynamics of the enzyme ...
It should then be possible to observe directly each of the dynamic events that result in catalysis. Time-averaged structural analyses can neither reveal the dynamics of catalysis nor describe the conformational details of molecules who structures-particularly of the active centre-are motile. Moreover,...
Oscillating chemical reactions, spinodal decomposition under shear flow, polymer growth and diffusion limited enzyme catalysis are all examples where the dynamics are influenced by the hydrodynamics of the condensed fluid. 展开 关键词: Theoretical or Mathematical/ catalysis chemical reactions chemically ...
摘要: The effects of structural changes on the activity of enzymes have been extensively studied. However, previous authors concentrated largely on the modification of the primary structures of the enzymes. On the other hand, although the importance of conformational integrity for enzyme activity is ...
Relaxation dispersion indicates rigid-body motion of the N-terminal domain and flexible dynamics in the I182–G209 loop, both at millisecond rates commensurate with kcat, implying that either might be rate limiting upon catalysis. Lipari-Szabo analysis indicates backbone flexibility on the nanosecond ...