在蛋白质折叠时与未折叠状态的溶剂相互作用的极性基团必须形成蛋白质内氢键以进行补偿,否则剥离水的巨大能源成本将不利于折叠。球状蛋白质结构的标志性特征来自以下考虑因素:α螺旋和β片二级结构,其中多肽骨架的极性羰基和酰胺基团可以形成氢键,以这样的方式组装,即非极性侧链像拼图游戏的碎片一样组合在一起,形成密集堆...
Proteins mediate the critical processes of life and beautifully solve the challenges faced during the evolution of modern organisms. Our goal is to design a new generation of proteins that address current day problems not faced during evolution. In contrast to traditional protein engineering efforts, ...
There are 20200 possible amino-acid sequences for a 200-residue protein, of which the natural evolutionary process has sampled only an infinitesimal subset. De novo protein design explores the full sequence space, guided by the physical principles that u
2016年,美国科学院院士、华盛顿大学医学院的David Baker教授在 The coming of age of de novo protein design 一文中写到: There are20200possible amino-acid sequences for a 200-residue protein, of which the natural evolutionary process has sampled only an infinitesimal subset. De novo protein design ex...
There are 20(200) possible amino-acid sequences for a 200-residue protein, of which the natural evolutionary process has sampled only an infinitesimal subset. De novo protein design explores the full sequence space, guided by the physical principles that underlie protein folding. Computational method...
The ability to design efficient enzymes from scratch would have a profound effect on chemistry, biotechnology and medicine. Rapid progress in protein engineering over the past decade makes us optimistic that this ambition is within reach. The development
The patch pump design (exemplified by Omnipod [Insulet, Billerica, MA, USA]) has a reservoir unit that adheres directly to the user’s skin and houses an integrated infusion set and automated inserter, thus making it ‘tubing-free’. Sensor-augmented insulin pumps (e.g. MiniMed Paradigm ...
As this prodrug design was originally not intended for lung delivery, debate is ongoing on whether respiratory viruses such as SARS-CoV-2 that predominantly infect alveolar epithelial cells might be better inhibited by nucleoside prodrugs that could be selectively converted to the active drug in the...
[180,181]. The role of mosaic de novo mutations is not yet fully appreciated, and it is to be expected that our understanding of this class of mutations will increase rapidly in the coming years because of further technological improvements as well as access to DNA from other (affected) ...
The coming of age of de novo protein design. Nature 537, 320–327 (2016). Article ADS CAS PubMed Google Scholar Bai, X. C., Martin, T. G., Scheres, S. H. & Dietz, H. Cryo-EM structure of a 3D DNA-origami object. Proc. Natl Acad. Sci. USA 109, 20012–20017 (2012). ...