Validation of the bicinchoninic acid (BCA) method for quantification of BSA solution (means ± S.D., N = 5).Yusuf K. DemirZafer AkanOya Kerimoglu
1. Introduction The bicinchoninic acid (BCA) assay, first described by Smith et al. (1) is similar to the Lowry assay, since it also depends on the conversion of Cu2+ to Cu+ under alkaline conditions (see Chapter 2). The Cu+ is then detected by reac-tion with BCA. The two assays ...
Protein concentrations were determined using the Bicinchoninic acid reaction (Pierce™ BCA Protein Assay Kit, ThermoFisherScientific, Waltham, Massachusetts, USA) according to the manufacturer’s instructions. CXCL7 and CXCL12 were quantified using the Pro-Platelet Basic Protein (Chemokine (C-X-C ...
Carlsbad CA). Output data was expressed as fold-induction over control condition following normalization toHPRT1, using Pfaffl’s method54. The qPCR primer sequences used forPRNP, LUMAN, HSPA5, andHPRT1are listed inTable 1.
RIPA buffer was used to extract the total protein, and the protein concentration was determined using the bicinchoninic acid method. Equal amounts (10–20 μg) of the protein were added to the sample wells. Sodium dodecyl sulfate-polyacrylamide gels (8–15%) were used to separate proteins, ...
Membrane protein was quantified by the bicinchoninic acid method (Pierce Chemical Co., Rockford, IL, USA) using bovine serum albumin dilutions as standard. Binding experiments were performed with membrane suspensions (0.2 mg of protein/ml) at room temperature in 50 mM Tris-HCl buffer, pH 7.4,...
The protein concentration was determined with the bicinchoninic acid method (Sigma). After trichloroacetic acid/aceton precipitation protein isolated from inoculated plants with water or Fol007 was dissolved in sample buffer at equal concentration (1.5 μg/μl) and 30 μl per sample was loaded on ...
Lysates were centrifuged at 16,000 g for 20 min at 4°C, and the supernatant was considered the whole cell extract, which was assayed for protein content using the bicinchoninic acid method (BCA; Pierce, Rockford, IL). Whole cell extracts were appropriately diluted in 3 X concentrated ...
The total protein levels of the lysates were determined using the bicinchoninic acid method. Protein extracts were analyzed using 8% sodium dodecyl sulfate polyacrylamide gel electrophoresis followed by transfer to the nitrocellulose membrane before staining with primary antibodies (Supplementary Table 8). ...
The mixture was treated ultrasonically for 20 min, and after centrifugation at 12,000×g at 4 °C for 20 min, the concentration of protein was determined using the bicinchoninic acid (BCA) method with a BCA Protein Assay Kit (Thermo, MA, USA). Protein quantification was performed according ...