The bicinchoninic acid (BCA) assay is a protein quantification technique that was first demonstrated by Paul K. Smith at the Pierce Chemical Company in 1985 (Smith et al., 1985). The principle of this technique is quite similar to the Lowry protein assay, which is one of the alternative pr...
Principle of Bicinchoninic Acid (BCA) Protein Assay The Bicinchoninic Acid (BCA) protein assay is a widely used method for the quantification of total protein in a sample. The BCA assay relies on the reduction of Cu2+ions to Cu1+ions by protein in an alkaline medium. The Cu1+ions then ...
Bicinchoninic Acid (BCA) Protein Assay (Smith) The bicinchoninic acid (BCA) assay is available in kit form from Pierce (Rockford, Ill.). This procedure is very applicable to microtiter plate methods. The BCA is used for the same reasons the Lowry is used. Stoscheck (1990) has suggested ...
世联博研公司现货供应UNITED STATES BIOLOGICAL品牌订货号为219448的BCA Protein BioAssay™ Kit, Reducing Agent Compatible (Bicinchoninic Acid) from UNITED STATES BIOLOGICAL产品
In this proof-of-principle study, we demonstrate that bicinchoninic acid (BCA) and copper, when combined with purine-specific chemical cleavage reactions, can be a colorimetric probe for the identification and quantification of adenosines and/or guanosines in single-stranded DNA oligomers, even in ...