1994. The Bicinchoninic Acid (BCA) Assay for Protein Quantitation. In Basic Protein and Peptide Protocols, John M Walker (Ed.). Humana Press, Totowa, NJ, 5-8.WalkerJ M. The bicinchoninic acid (BCA) assay for protein quantitation[J]. Methods Mol Biol , 1994. 32: 5....
1. Introduction The bicinchoninic acid (BCA) assay, first described by Smith et al. (1) is similar to the Lowry assay, since it also depends on the conversion of Cu2+ to Cu+ under alkaline conditions (see Chapter 2). The Cu+ is then detected by reac-tion with BCA. The two assays ...
Bicinchoninic acid assay Hydrolysis of carbohydrates was assessed in vitro by measuring the accumulation of reducing sugar ends using the BCA assay42 and D-glucose as a standard for calibration (Supplementary Fig. 11). Purified enzyme was diluted to 1 mM in 20 mM (N-2-hydroxyethylpiperazine...
The concentration of total protein was determined by using the Bicinchoninic Acid Protein Assay Kit (BCA1, B9643, Sigma-Aldrich) according to the manufacturer’s instructions. Transmission electron microscopy The mouse peritoneal cells were pelleted by centrifugation at 3000 r.p.m. and then ...
The total membrane protein concentration of the vesicles was determined using the bicinchoninic acid assay (Thermo Scientific Pierce, Rockford, IL) according to the manufacturer’s protocol. Transport measurements were performed at the indicated pH values (ranging between pH 6.5 to 9.75) at 25°C ...
Protein concentrations in the animal microsomal fraction were determined using the bicinchoninic acid protein assay with bovine serum albumin as a standard [40]. Human hepatic microsomes (pooled sample) (male sample LOT: 3043885, 36635; female sample LOT: 5061002) were from Corning® Gentest™...
The eluted protein was analyzed by 12% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), passed through a desalting gravity column (Sangon, Shanghai, China), eluted in PBS, and the concentration was determined using a bicinchoninic acid (BCA) assay kit (Cwbio, Shanghai, ...
A portion of the lysates was cleared to quantify total protein using the bicinchoninic acid (BCA) protein quantification assay (Sigma-Aldrich). A volume corresponding to three mg of total protein was transferred to glass tubes and lyophilised overnight (Virtis). One hundred microliters of 1 ...
(Roche, Penzberg, Germany). After centrifugation at 13,000 × gat 4 °C for 20 min, supernatant was collected. Protein was quantified with the Bicinchoninic Acid Assay kit (Pierce, Rockford, IL). For immunoblot analysis, protein samples were loaded onto sodium dodecyl sulfate-...
To control for this issue, all hearts had their protein quantified by the bicinchoninic acid protein assay method. The protein concentration was used to normalize values of UDP-GlcNAc concentration and glycolytic efflux. Statistical analysis All reported values are mean ± S.E. A two-way, ...