The bicinchoninic acid (BCA) assay, first described by Smith et al. (1) is similar to the Lowry assay, since it also depends on the conversion of Cu2+ to Cu+ under alkaline conditions (see Chapter 2). The Cu+ is then detected by reaction with BCA. The two assays are of similar ...
1. Introduction The bicinchoninic acid (BCA) assay, first described by Smith et al. (1) is similar to the Lowry assay, since it also depends on the conversion of Cu2+ to Cu+ under alkaline conditions (see Chapter 2). The Cu+ is then detected by reac-tion with BCA. The two assays ...
Bicinchoninic acid assay Hydrolysis of carbohydrates was assessed in vitro by measuring the accumulation of reducing sugar ends using the BCA assay42 and D-glucose as a standard for calibration (Supplementary Fig. 11). Purified enzyme was diluted to 1 mM in 20 mM (N-2-hydroxyethylpiperazine...
The clarified lysate was transferred to a new tube and protein content was determined by bicinchoninic acid (BCA) assay. Trichloroacetic acid (TCA) was added to the remaining lysate to a final concentration of 10% and spun down at 12,000 × g for 5 min, 4 °C. The supernatant...
The eluted protein was analyzed by 12% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), passed through a desalting gravity column (Sangon, Shanghai, China), eluted in PBS, and the concentration was determined using a bicinchoninic acid (BCA) assay kit (Cwbio, Shanghai, ...
Briefly, cells were grown for 3 days in 25-cm2 flasks, collected in 1 ml medium and 100 μl used for quantification using the BCA (bicinchoninic acid) protein assay kit (Pierce, see below). Same amounts of total protein (1 μg) were aliquoted per well in a 96-multiwell plate in ...
(pH 7.8). High-MAb-containing fractions were identified using the bicinchoninic acid (BCA) protein assay (BCA-1, Sigma-Aldrich, USA), pooled and repeatedly dialysed against 2 litre volumes of 0.2 M NaHCO3/0.5 M NaCl (pH 8.9). Cyanogen-bromide-activated Sepharose 4B (0.8 g) (C9142, ...
BCA Bilan de Compétences Approfondi (French: Thorough Review of Skills; employment assessment) BCA Business Corporation Act BCA Brethren Colleges Abroad (foreign exchange program) BCA Belle Chasse Academy (Belle Chasse, LA) BCA Bunda College of Agriculture (Malawi) BCA Bicinchoninic Acid Assay BCA ...
The protein concentration in the tissue lysate was determined using the bicinchoninic acid (BCA) protein assay kit (Thermo Scientific, Rockford, IL, USA) [24]. Protein (20 µg) was resolved by 4–15% gradient SDS-PAGE gel (Biorad, Hercules, CA, USA). The blots were then transferred ...
Bicinchoninic acid (BCA) assay reagents and standards were obtained from Pierce (Rockford, IL); and purified, deionized water, >18 MΩ, (Nanopure Infinity ultrapure water system, Barnstead, Dubuque, IA) was used to make all aqueous buffers. Dissected lung tissues were directly frozen at −80...