其次,两个分子的bicinchoninic acid (BCA) 络合一个一价铜离子 (Cu+) ,形成一种在562 nm处有强吸收值的紫色复合物。本制品依此为原理,根据测定562 nm处光吸收值,与标准曲线对比,获得待测蛋白的浓度定量。使用BCA法进行蛋白质定量具有以下特点:1. 不受蛋白质种类的影响,在0.02~2 mg/ml浓度范围内蛋白质浓度...
Following removal of isopropanol, a Pierce BCA (bicinchoninic acid assay) protein assay (Thermo Fisher Scientific) was performed on the tissue culture wells to determine the cellular protein concentration. Tri-one dimensional thin-layer chromatography (TOD-TLC) Alveolar macrophages (AMs) were isolated ...
The Bicinchoninic Acid (BCA) Protein Assay is a detergent-compatible formulation based on bicinchoninic acid (BCA)for the colorimetric detection and quantitation of total protein. This method combines the well-known reduction of Cu+2 to Cu+1 by protein in an alkaline medium (the biuret reaction)...
Cre recombinase is split into two fragments, with the N-terminal Cre fragment fused to a Coh2 domain; this CreN-Coh2 fusion protein is constitutively expressed. The C-
(bicinchoninic acid,BCA)法蛋白质定量试剂盒购自江苏凯基生物技术股份有限公司,transwellTM小室购自美国Corning公司,Matrigel基质胶购自美国BD Biosciences公司,细胞计数试剂盒-8(cell counting kit-8,CCK-8)购自上海碧云天生物技术有限公司,TRI...
Protein content was measured with a Bicinchoninic Acid Protein Assay Kit (Pierce Chemical, Rockford, IL). The methods used in the assay for glucuronidation... Y Ota,Y Maruo,K Matsui,... - 《Pediatric Research》 被引量: 28发表: 2011年 加载更多研究...
stored at –70°C until use. Protein concentration was determined using a bicinchoninic acid protein assay kit (Pierce, Rockford, IL). rPmp1 was visualized as a 17.9-kDa protein by Coomassie stain following SDS-PAGE and immunoblotting using a pool of serum from patients with high titers to ...
The protein concentration was determined using a bicinchoninic acid (BCA) protein assay kit (Beyotime Institute of Biotechnology), with bovine serum albumin as the standard. The T-AOC and SOD contents were calculated, and the results were expressed as activity per mg of protein. 2.9. Cell ...
Briefly, cells were grown for 3 days in 25-cm2 flasks, collected in 1 ml medium and 100 μl used for quantification using the BCA (bicinchoninic acid) protein assay kit (Pierce, see below). Same amounts of total protein (1 μg) were aliquoted per well in a 96-multiwell plate in ...
column (1 mL; GE Healthcare) in line with the ÄKTA start (GE Healthcare) according to the standard operating procedure. Purified proteins were dialyzed in 20 mM HEPES buffer (pH 7.0). Protein concentrations were determined using a Bicinchoninic Acid Protein Assay kit (Takara Bio Inc.)...