AmyP is 639 amino acids in length and has a theoretical molecular weight of approximately 70 kD. It has been proposed to contain a conserved catalytic domain at the N terminus2 and a starch binding domain at the C terminus3. AmyP exhibits high substrate specificity for starch over ...
The isolated H2A-H2B heterodimer comprises 256 amino acids with long disor- dered tails, which is big enough for the CS-Rosetta calculation22; as a result, the Rosetta protocols, AbinitioRelax23 and FloppyTail24, were used to obtain structures of H2A-H2B in isolation. The calculated ...
Introduction Alzheimer's disease (AD) is the most common cause of senile dementia associated with progressive disability. The inherited disease, in an autosomal dominant way, generally leads to a lethal outcome after about 5–10 years from the onset of the first symptoms [1]. Its pathology is...
The mutation of L124 with an isoleucine slightly increased the selectivity of the ArM for the γ-lactam 2b (76% e.e. (S)), while a glycine residue favors the β-lactam 2a (62% e.e. (─., identified as the (R) enantiomer by VCD experiments) (Scheme 1). ...
We tested a rational supply of amino compounds ((±)-3-amino-2-piperidinone, glycine, and L-threonine) in the culture of an isoindolinone high-producing marine fungus, Stachybotrys longispora FG216. As a result, we obtained four new kinds of isoindolinone derivatives (FGFC4–GFC7) by ...
Therefore, it is often used as a reference for gene expression and protein research [4]. However, GAPDH has recently been shown to play a vital role in many cellular processes, like energy production, DNA repair, transcriptional regulation, sugar and amino acid balance, embryo development, ...
The VH/VHH-bound phages were eluted with 0.2 M glycine-HCl (pH 2.2) and the pH was brought up immediately by adding a few drops of 2 M Tris-base solution. The phages from each well were inoculated into 20 mL of log phase-grown ER2738-E. coli and incubated at 37 ˝C for 4 h....
membranes Article A Novel Proline-Rich Cathelicidin from the Alpaca Vicugna pacos with Potency to Combat Antibiotic-Resistant Bacteria: Mechanism of Action and the Functional Role of the C-Terminal Region Pavel V. Panteleev * , Victoria N. Safronova, Roman N. Kruglikov, Ilia A. Bolosov, ...
TMV is a systemic plant viral pathogen, and there are no effective control methods or treatments available to protect plants against TMV infection. At present, some commercial agents, such as ningnanmycin and ribavirin, exhibit unsatisfactory antiviral activities [1–4]. Therefore, it is still ...
As the synthesis of nucleic acids evolved from β-linked polyesters, the singlet coding system for replication evolved into a four nucleotide/four amino acid process (AMP = aspartic acid, GMP = glycine, UMP = valine, CMP = alanine) and then into the triplet ribosomal process that permitted ...