AccelerRT® 5G逆转录酶 (RNase H-)是基于MMLV-RT酶通过试管进化(in vitro evolution)而产生的一种新型逆转录酶,该酶具RNA和DNA依赖性的聚合酶活性,而不具有RNase H活性。具有高效模板转换功能,可用于全长cDNA合成。与野生型MMLV RT相比,该工程化酶具有更显著的热稳定性、合成持续性以及合成效率。
However, care should be taken in the annotation of alternative transcripts, especially when the RT used is poorly thermostable and when the putative intron is flanked by direct repeats, which are the substrate for template switching.doi:10.1016/j.ygeno.2005.12.013...
murine leukemia virus RT to add non-templated C residues to the 3 end of a completed cDNA, which can then base pair and enable template switching to the 3 end of an adapter DNA This work was supported by National Institutes of Health Grants R01 GM37949 (toA. M. Lambowitz) andR35 GM...
Group II intron RTs have an end–to–end template-switching activity similar to that of MRP and R2 element RTs (6, 39), and this activity has been utilized for adapter addition in RNA-Seq using a thermostable group II intron RT (TGIRT-seq) (6, 8, 9). Unlike the R2 element RT (...
In this study, we describe the application of nucleotide isomers that form non-standard base pairs in the template switching oligo to prevent background cDNA synthesis. When such bases are added to the 5' end of the template switching (TS) oligo, they inhibit MMLV-RT from extending the cDNA...
For each of these templates, you can control the visibility by switching between the statuses Closed and Open. A closed template is still being worked on by the template application team and, therefore, can currently not be transferred to any other rollout project. Only templates that are open...
This technique enables custom template switching based on input data, at the application level. For more information, see DataTemplateSelector. Applies to TermékVerziók WinRT Build 10240, Build 10586, Build 14383, Build 15063, Build 16299, Build 17134, Build 17763, Build 18362, Build 19041, ...
Reprints and permissions Cite this chapter Zhu, Y.Y., Chenchik, A., Li, R., Hsieh, F.Y., Siebert, P.D. (2002). Construction of cDNA Libraries from Small Quantities of Total RNA Using Template Switching Catalyzed by M-MLV Reverse Transcriptase. In: Bird, R.C., Smith, B.F. (eds...
This method is relatively rapid, but poly(dT) tailing and variable non-templated nucleotide addition by the retroviral RT to the 3′ end of the DNA product prior to template switching make it difficult to precisely identify the DNA template ends and can introduce strong bias for regions with ...
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