prefer initiating DNA synthesis from a predominantly A-form rather than a B-form duplex and also holds true for template switching. In the configuration used for TGIRT-seq, the enzyme initiates DNA synthesis by template-switching from a synthetic RNA/DNA heteroduplex and template switches with si...
AccelerRT® 5G逆转录酶 (RNase H-)是基于MMLV-RT酶通过试管进化(in vitro evolution)而产生的一种新型逆转录酶,该酶具RNA和DNA依赖性的聚合酶活性,而不具有RNase H活性。具有高效模板转换功能,可用于全长cDNA合成。与野生型MMLV RT相比,该工程化酶具有更显著的热稳定性、合成持续性以及合成效率。
We found that the +CAA product was the major species formed with either enzyme in the Smart-seq2 buffer (Fig. S4, B and D), which is consistent with the results obtained with Template Switching RT. In line with the observed low template switching efficiencies (Fig. S1A), the reverse ...
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The template switching PCR (TS-PCR) method of cDNA synthesis represents one of the most straightforward approaches to generating full length cDNA for sequencing efforts. However, when applied to very small RNA samples, such as those obtained from tens or
The latter leaves a 1-nt 3′ overhang (N) that can direct template switching by base pairing to the 3′ nucleotide (N′) of the acceptor nucleic acid (6). Upon the addition of dNTPs (an equimolar mix of dATP, dCTP, dGTP, and dTTP), GsI–IIC RT initiates reverse transcription by ...