APOBEC3 Host Restriction Factors of HIV-1 Can Change the Template Switching Frequency of Reverse Transcriptaseenzyme-DNA interactionsDNA synthesisproviral DNA integrationretroviral recombinationpolymeraseThe APOBEC3 family of deoxycytidine deaminases has the ability to restrict HIV-1 through deamination-...
AccelerRT® 5G逆转录酶 (RNase H-)是基于MMLV-RT酶通过试管进化(in vitro evolution)而产生的一种新型逆转录酶,该酶具RNA和DNA依赖性的聚合酶活性,而不具有RNase H活性。具有高效模板转换功能,可用于全长cDNA合成。与野生型MMLV RT相比,该工程化酶具有更显著的热稳定性、合成持续性以及合成效率。
phosphates, which block TGIRT template switching, and heat denatured. After pre-incubating the enzyme with template-primer substrate, a step that significantly increases the efficiency of the DNA synthesis (see Supplementary Fig.S2), the enzyme template-primer complex is added to the DNA to be seq...
We found that the +CAA product was the major species formed with either enzyme in the Smart-seq2 buffer (Fig. S4, B and D), which is consistent with the results obtained with Template Switching RT. In line with the observed low template switching efficiencies (Fig. S1A), the reverse ...
The template switching PCR (TS-PCR) method of cDNA synthesis represents one of the most straightforward approaches to generating full length cDNA for sequencing efforts. However, when applied to very small RNA samples, such as those obtained from tens or
R2 RT is highly processive and rapidly and efficiently extends to the end of the short RNA templates used in these assays (8). We have therefore attempted several methods to block polymerization to determine whether a stalled enzyme could be induced to jump between RNA templates. However, neithe...
Overview of the template-switching reaction and determination of saturating enzyme concentrations Fig. 1B depicts the assay that we used to analyze the template-switching activity of GsI–IIC RT, which is based on the method used for 3′-adapter addition in TGIRT-seq (6, 8, 9). In this ...
Evaluation of Molecularly Imprinted Polymers for Chlorpromazine and Bromopromazine Prepared by Multi-step Swelling and Polymerization Method-The Application for the Determination of Chlorpromazine and Its Metabolites in Rat Plasma by Column-switching LC. J. Pharm. Biomed. Anal. 2019, 174, 248–255. [...