A prerequisite for effective clonal cultivation or single-cell analysis is the efficient isolation of individual cells from liquid cell suspensions. This review provides an overview of technologies that are used to automate the isolation of single cells for subsequent cultivation or analysis. First, ...
methods for the integrative analysis of single-cell multiomics data. Introduction Recent advances in single-cell isolation and barcoding technologies have enabled DNA, mRNA, and protein profiles to be measured at a single-cell resolution. Various experimental protocols have been developed and applied ...
(C) Single B cell analysis (VH–VL paired). Primary B cells are encapsulated together with beads bearing barcoded-oligos and subsequently lysed, allowing the oligos to anneal with single-cell total mRNAs. Upon emulsion breaking, these oligos are then amplified and prepared for next-generation ...
Microfluidic technology (Fig.1e) for single-cell isolation has gained popularity due to its low sample consumption and low analysis cost together with the fact that it enables precise fluid control22. Importantly, the nanoliter-sized volumes required for this technique substantially reduce the risk o...
The global population is increasing, with a predicted demand for 1250 million tonnes of animal-derived protein by 2050, which will be difficult to meet. Single-cell protein (SCP) offers a sustainable solution. This review covers SCP production mechanisms, microbial and substrate choices, and advanc...
Stem cells are a heterogeneous cell population characterized by their ability of self-renewal and differentiation.Stem cell research at the cell population level can not meet the demand for further understanding the biology and its applications.Recently,the single cell-based technologies are rapidly appl...
isolation of single cells for subsequent studies. In the absence of techniques to separate persistent bacteria from other cell types, fluorescence-activated cell sorting (FACS) has become the gold standard for examining persisters’ physiology [52,149]. Mohiuddin and colleagues use fluorescent cell-...
Single-cell multi-omics technologies have emerged. They refer to the simultaneous measurement of various types of data in the same cell, allowing for an accurate and detailed depiction of the cellular state. Integrating single-cell transcriptomic sequencing with comprehensive multi-omics data to map ...
For example, single-cell genomics will help to uncover cell lineage relationships; single-cell transcriptomics will supplant the coarse notion of marker-based cell types; and single-cell epigenomics and proteomics will allow the functional states of individual cells to be analysed. These technologies ...
We will describe how microfluidics can address single-cell analyses for immune cells and how this technology already had an impact on the field of immunology. We would like to mention a review by Chattopadhyay et al.10 on single-cell technologies for monitoring immune cells, which covers the ...