Furthermore, coordinated CD4+ and CD8+ TCR-T cell response broadens and deepens clinical responses, necessitating additional incorporation of a CD8 coreceptor, thus raising payload and expression challenges in TCR-T construct design. Here, we screened a library of FAS-based SwRs in combination with...
Adoptive cell therapy with tumor-infiltrating lymphocytes (TIL) can mediate tumor regression, including complete and durable responses, in a range of solid cancers, most notably in melanoma. However, its wider application and efficacy has been restricted
Development of production technology The development of production technology encompasses all stages, from selecting a relevant antigen (AG) for TCR design and the corresponding genetic construct, to evaluating the efficiency of transducing selected cells with the synthesized construct (Fig.2). Fig. 2 ...
TCR-construct-inherent differences in surface expression of Mel15’s neoTCRs in retrovirally and orthotopically TCR-engineered T cells All cells originating from patient PBMCs possess a certain differentiation state at the time of blood collection due to multiple variables, including potential previous...
The development of production technology encompasses all stages, from selecting a relevant antigen (AG) for TCR design and the corresponding genetic construct, to evaluating the efficiency of transducing selected cells with the synthesized construct (Fig.2). ...
Naturally evolved T-cell receptors (TCRs) exhibit remarkably high specificity in discriminating non-self antigens from self-antigens under dynamic biomechanical modulation. In contrast, engineered high-affinity TCRs often lose this specificity, leading t
NOVEL TECHNOLOGY Silence™ CAR-T Cell: A novel platform to enhance CAR-T cell immunotherapy by combining RNAi technology to suppress genes that may impede CAR functionality. LEARN MORE NOVEL TECHNOLOGY NEW SOLUTION Canine CAR-T Therapy Development: From early target discovery, CAR design and cons...
The authors also thank Dina Schneider for providing the CD33 CAR construct as well as Lilian Andrea Martinez-Carrera and Stefan Tomiuk for supporting the RNA-seq analysis. Furthermore, the authors acknowledge the support of Marvin Markel, Lennart Wessels, and Marek Wieczorek for the Tetramer ...
Other approaches include the T cell receptor fusion construct (TRuC) that covalently links the antigen-binder to CD3ε9, and the T cell antigen coupler (TAC)10, which is described below. The TAC receptor (Fig. 1A) contains three functional domains: (1) a targeted, though interchangeable ...
A similar study using a PD-1:CD28 CSR construct showed that its co-expression is equally beneficial for CD4+ and CD8+ T cells, and can improve the functional avidity of TCRs targeting tyrosinase in xenograft models of human melanoma [219]. Enhanced in vivo anti-tumor activity and cytokine ...