采用Beckman Coulter Beta Mark TCR Vβ Repertoire Kit®,包含8管预混抗体,覆盖24种TCR-Vβ家族(占正常人类Vβ库的70%)。 每管含三种不同荧光标记(FITC、PE、双标)的抗体,通过8个FITC/PE点图分析Vβ家族分布。 设门策略与数据解读 单族...
T cell receptor beta chain (TCR-Vβ) repertoire of circulating CD4+ CD25−, CD4+ CD25low and CD4+ CD25high T cells in patients with long-term renal allograft survivaldoi:10.1111/j.1432-2277.2009.00946.xkidney transplantregulatory T cellsT cells...
SMARTer Human TCR a/b Profiling Kit v2—flexible kit with ultimate sensitivity for understanding the TCR repertoireNOTE: We recommend the reconfigured versions of the kits below, SMART-Seq Human TCR (with UMIs) (Cat. # 634780, 634781 & 634779). SMARTer Human TCR ...
在疾病进程中,TCR V和J基因的使用频率偏好性可能出现显著变化。这是因为疾病状态下,某些特定的T细胞克隆可能因为针对某种抗原的高亲和力而被强烈激活和扩增,从而导致某些V或J基因的使用频率增加。例如,在某些类型的肿瘤中,已发现存在肿瘤特异性T细胞克隆的富集,表现为TCR V和J基因使用频率的非随机性分布。另一方面,在...
The aim of the present study was to assess by comparative flow cytometric analysis the peripheral blood CD4+ TCR Vβ repertoire of children with an organ-specific autoimmune disorder, such as type 1 diabetes mellitus (T1DM), in comparison to children with a systemic autoimmune disease, such as...
1. Association between the HLA haplotype and the TCR-V beta repertoire of anti-hCMV specific memory T-cells in immunocompetent healthy adults [J] . Rodriguez-Caballero A, Garcia-Montero AC, Almeida J, Cytometry, Part B. Clinical cytometry: the journal of the International Society for Analyti...
四、组库的重叠分析(Repertoire overlap analysis) tcR提供了许多基于clonotypes之间共享的cloneset来评估相似度的函数,处理数据框数据。 1. 重叠量化(Overlap quantification) repOverlap 函数是计算cloneset重叠系数的所有函数的通用接口 (1)共享克隆型的数量
Analyz- ing the CDR3 repertoire with respect to TCR-Beta chain V-D-J and V–J rearrangements in peripheral T cells using HTS. Sci Rep. 2016;6:29544. 13. Schober K, Buchholz VR, Busch DH. TCR repertoire evolution during maintenance of CMV-specific T-cell populations. Immunol Rev. 2018...
Home›Learning centers›Next-generation sequencing›Technical notes›Immune Profiling›TCR repertoire profiling from human samples (single cells) Technical notes DNA-seq Immune Profiling Track B-cell changes in your mouse model Efficient and sensitive profiling of human B-cell rec...
each encoded by distinct gene segments. TCR Vβ2 is a variant of the TCR β chain. α/β T cells express a diverse α/β TCR repertoire that specifically co-recognizes self or foreign antigen bound to antigen-presenting molecules, which thereby leads to T cell–mediated immunity. For exampl...