RGEN-seq for highly sensitive amplification-free screen of off-target sites of gene editors Article Open access 08 December 2021 OliTag-seq enhances in cellulo detection of CRISPR-Cas9 off-targets Article Open access 06 June 2024 Improving the sensitivity of in vivo CRISPR off-target detecti...
Corey. Predicting potential miRNA target sites within gene promoters. Bioorganic & Medicinal Chemistry Letters 2009; 19: 3791-3794... ST Younger,A Pertsemlidis,DR Corey - 《Bioorganic & Medicinal Chemistry Letters》 被引量: 96发表: 2009年 Multi-target-directed coumarin derivatives: hAChE and BAC...
Putrescine and other polyamines are required for the division of normal cells. LOSS OF HETEROZYGOSITY (LOH). In cells that carry a mutated allele of a tumour-suppressor gene, the gene becomes fully inactivated when the cell loses a large part of the chromosome carrying the wild-type allele. ...
It controls lysosomal function via the coordinated lysosomal expression and regulation (CLEAR) gene network (including genes coding for hydrolases, lysosomal membrane proteins and the proton pump v-ATPase complex), and additional lysosome-related processes such as autophagy, endocytosis and exocytosis. ...
This work provides the practical basis for potential applications of targeted group II introns in genetic engineering, functional genomics, and gene therapy. 展开 关键词: Humans Lactococcus lactis Proviruses Cell Line Escherichia coli RNA, Catalytic DNA DNA, Viral Receptors, CCR5 Gene Therapy ...
The possibility of editing the genome of an organism, inserting, deleting or modifying DNA sequences at targeted loci, holds great promise for the advancement of gene therapy in clinical populations. In 1990, the FDA approved the very first US-based gene therapy-related clinical trial in which ...
Finally, 8% of primary colorectal tumors were found to harbor a loss of function mutation in one allele of the PPARγ gene emphasizing a putative role for this receptor as a tumor suppressor in humans (27). PPARγ may also be important in modulating the intestinal inflammatory response. ...
Antibody targeted gene transfer to endothelium. J Gene Med 5:311-323 One of the drawbacks of the currently available vectors for gene therapy is the lack of selectivity in gene delivery. We have therefore investigated a strategy to generate immunoliposomes to target non-viral vectors to cell ...
The pathogenic gene sites in CHIP of these patients were distributed in the U-box and TPR domains and even in the CC domain, suggesting that all three domains are indispensable in maintaining CHIP function. The clinical symptoms of SCAR16 are complex; in addition to early-onset progressive ...
defining the target prioritization landscape for 30 immune traits at the gene and pathway level. We demonstrate how our genetics-led drug target prioritization approach (the priority index) successfully identifies current therapeutics, predicts activity in high-throughput cellular screens (including L1000,...