We have developed conditions that permit the use of the SYBR Green I dye in PCR with little PCR inhibition and increased sensitivity of detection compared to ethidium bromide. Additionally, we havenewer SYBR Greendyes that fluoresce more brightly and inhibit PCR less than the o...
We have developed conditions that permit the use of the SYBR Green I dye in PCR with little PCR inhibition and increased sensitivity of detection compared to ethidium bromide. Additionally, we havenewer SYBR Greendyes that fluoresce more brightly and inhi...
Sensitivity of each CHIKV RT-PCR assay: SYBR Green and Taqman probe-based on PFU per reaction and PFU/ml of cell-culture supernatants of CHIKV D67Y08 and D1225Y08.Grace YapKwoonYong PokYeeLing LaiAngela ChowYeeSin...
Results obtained from SYBR Green PCR Arrays show that 83 out of the 90 selected genes (92%) are considered to be present in both samples A and B, which is similar to the values reported in the MAQC study for the detection sensitivity of the other three quantitative platforms [8]. Eighty...
Finding Multiple Melt-Curve Peaks When Using SYBR Green in Real-Time PCR - Ask TaqMan - Ep. 8 The causes of multiple peaks in a melt curve when using SYBR Green dye in Real-Time PCR. SYBR Green I chemistry is a free-floating dye. Here's how it works. ...
Briefly, potential primer pairs were initially tested in the LightCycler by the fluorescent dye SYBR Green I (Roche Biochemicals). The optimum primer pair was selected based on specificity (a single, appropriately sized amplicon) and efficiency of amplification (lowest Ct value which is defined as ...
the TaqMan real-time PCR described here is less likely to produce a false positive than a conventional PCR assay or the SYBR Green I-based real-time PCR. TaqMan-based real-time PCR may be more specific than SYBR Green I-based real-time PCR because the former requires specific probes that...
Amongst the 3 systems tested, the best compromise was obtained with the SYBR Green assay, which, by comparison with our probe-based assay provided an improved sensitivity without altering the detection specificity. Interestingly, a majority of probe-negative/BOXTO- or SYBR Green-positive samples ...
ORF1ab and 2019_nCoV_N3 were the best combination for sensitive and reliable SARS-CoV-2 molecular diagnostics due to their high sensitivity, specificity, and broad accessibility. With suitable primer sets, the SYBR Green method performs better than the TaqMan one. With suitable primer sets, both...
SYBR Green I chemistry is a free-floating dye. Here's how it works. Phases of Real-Time PCR and Why They're Important – Ask TaqMan Episode 9 But it's normally difficult to see PCR's phases in action. That said, once we do real-time PCR, we can easily...