SYBR Green 引物 SYBR Green 预混液 TaqMan 检测试剂盒 TaqMan 预混液 * 取决于模板质量和引物/设计优化。 图1:基于 TaqMan 和基于 SYBR Green 的检测工作流程比较。 TaqMan 方法 背景 最初,使用嵌入式染料进行实时荧光PCR产物定量。但这些染料存在重大缺点,即会同时检测...
Figure 1:Comparison of TaqMan-based and SYBR Green–based detection workflows. TaqMan chemistry Background Initially, intercalator dyes were used to measure real-time PCR products. The primary disadvantage to these dyes is that they detect accumulation of b...
Maeda, H, Fujimoto, C, Haruki, Y, Maeda, T, Kokeguchi, S, Petelin, M, Arai, H, Tanimoto, I, Nishimura, F, Takashiba, S (2003) Quantitative real-time PCR using TaqMan and SYBR Green for Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, tetQ gene ...
以12 株T0 转基因水稻为材料,分别用TaqMan 与SYBR Green 实时定量PCR 法检测其拷贝数,然后用SAS 9.1 软件对2 种方法的结果进行t 检验分析。通过SAS 对2 组数据的t 检验分析,在内参基因扩增效率与目的基因扩增 效率接近时,SYBR Green 与TaqMan 探针法的结果接近,差异不显著;当内参基因与目的基因扩增效率差异明显...
Figure 1: Comparison of TaqMan?- and SYBR? Green-Based Detection Workflows. TaqMan? 试剂 背景 起初,使用嵌入染料检测实时荧光定量PCR产物。 这些染料的主要缺点在于它们会同时检测特异性和非特异性PCR产物的积聚。 Development of TaqMan? Chemistry 实时荧光定量PCR系统的改进得益于荧光标记探针的问世,其采用了Taq...
The causes of multiple peaks in a melt curve when using SYBR Green dye in Real-Time PCR. SYBR Green I chemistry is a free-floating dye. Here's how it works. Phases of Real-Time PCR and Why They're Important – Ask TaqMan Episode 9 ...
Detection and identification of Anaplasma marginale by real-time PCR; 实时荧光PCR检测牛边缘无浆体方法的建立及应用 3. Rapid identification of Bactrocera latifrons by real-time PCR using SYBR Green chemistry; SYBR Green实时荧光PCR快速鉴定辣椒实蝇 更多例句>> 补充...
Assays were designed to the β-tubulin gene, the intergenic spacer (IGS) region of the nuclear ribosomal DNA and also to a previously published, species-specific sequence characterised amplified region (SCAR) marker; the assays were compared to a published method based on SYBR Green I technology...
Within a single thermocycler run, mycobacterial 16S rDNA and the Mycobacterium tuberculosis global lineage-defining RD750 polymorphism are targeted in separate reaction tubes, each of which includes both TaqMan and SYBR Green chemistries. The results of this 16S-RD assay differentiate M. tuberculosis ...
Real-time PCRGMO detectionTaqManSYBR greenMolecular beaconsAlternative chemistriesSeveral techniques have been developed for detection and quantification of ... Meti Buh Gapari,T Tengs,JLL Paz,... - 《Analytical & Bioanalytical Chemistry》 被引量: 178发表: 2010年 加载更多来源...