SYBR Green 预混液 TaqMan 检测试剂盒 TaqMan 预混液 * 取决于模板质量和引物/设计优化。 图1:基于 TaqMan 和基于 SYBR Green 的检测工作流程比较。 TaqMan 方法 背景 最初,使用嵌入式染料进行实时荧光PCR产物定量。但这些染料存在重大缺点,即会同时检测特异性以及非特异...
SYBR Green–based detectionTaqMan-based detection UsesSYBR Green dye(a dsDNA binding dye) to detect PCR product as it accumulates during PCR. Uses afluorogenic probespecific to target gene to detect target as it accumulates during PCR. SpecificityLower*Hi...
Combination of SYBR Green II and TaqMan Probe in the adulteration detection of Dendrobium devonianum by fluorescent quantitative PCR. Int J Food Sci Tech 2016;50:2572-8.Yan, W.J.; Zhang, J.Z.; Zheng, R.; Sun, Y.L.; Ren, J.; Ding, X.Y. Combination of SYBR Green II and TaqMan...
The discrepancies in the expression results for the remaining three genes (ABCD1, CDK5R1 and IGFBP5) cannot simply be explained by the difference in probe locations. Interestingly, while these three genes display discordance among the microarray platforms, all four quantitative platforms show comparable...
Finding Multiple Melt-Curve Peaks When Using SYBR Green in Real-Time PCR - Ask TaqMan - Ep. 8 The causes of multiple peaks in a melt curve when using SYBR Green dye in Real-Time PCR. SYBR Green I chemistry is a free-floating dye. Here's how it works. ...
Beacon Designer™is the only commercially available program with uses innovative proprietary algorithms to design optimal primer-probe sets for single tube multiplex assays using TaqMan® for up to 4 targets. All sets are analyzed for cross compatibility and cross homology to other targets to ensur...
A TaqMan-based real-time polymerase chain reaction (PCR) assay was devised for the detection of porcine parvovirus (PPV). Two primers and a TaqMan probe for the non-structural protein NS1 gene were designed. The detection limit was 1 × 102 DNA copies/μ
Briefly, both forward and reverse primer sites and a TaqMans probe site of the Bacillus anthracis protective antigen gene was mutated to create a novel sequence with no known homology to published sequences with a resulting amplicon of 153 bp. The IPC was shown to be sensitive to a variety ...
2.2 TaqMan荧光定量PCR条件优化及标准曲线的绘制根据KAPA Probe Fast Universal qPCR 试剂盒说明书确定反应体系为20 μL,经过优化最终确定为qPCR Mix 10 μL、探针(10 μmol/L)0.6 μL、上/下游引物(10 μmol/L)0.8 μL、Rox 0.4 μL、模板1 μL,dd H2O 补至20 μL。经过对反应条件的优化,确定最佳反应...
TaqMan probeThe aim of the study was the application and comparison of real-time PCR methods based on the fluorescence of SYBR Green I intercalating dye and TaqMan probes for the detection of the 23S rDNA gene of Listeria spp. and the hlyA gene of Listeria monocytogenes. Five strains of L...