优化的 TaqMan® 探针和 PCR 引物组:TaqMan® 测定为开箱即用型。Life Technologies 的科学家已开发了较全面的实时荧光定量 PCR 测定系列,可用于基因表达、microRNA、非编码 RNA、蛋白质分析、突变检测、拷贝数变异和 SNP 基因分型。从涵盖 23 个物种的超过 800 万个预设计测定中进行选择。如果我们未提供适合...
Life Technologies 出版物 研究资料:在单个平台上同时分析 microRNA 和蛋白表达 学术海报:一种基于定量 PCR 的新型蛋白检测方法 学术海报:适用于干细胞中蛋白标志物的检测和相对定量之使用 qPCR 的新型蛋白表达定量 学术海报:用于监测人多能细胞...
Today we are launching Life Technologies’TaqMan® Assays QPCR Guarantee Program, which is designed to provide customers with unparalleled peace of mind by offering to replace any of our more than 7 million pre-designed TaqMan® assays that don’t meet their expectations (see video). ...
一年前,Life Technologies公司宣布推出TaqMan® Mutation Detection Assays。这是一个高度灵敏且新颖的研究工具,能够检测到隐藏在100万个正常拷贝的背景之下的单个突变分子。如今,Life Tech再次扩展其突变检测分析,可检测另外21个癌基因中241个较常见的突变。 TaqMan® Mutation Detection Assays可实现异质样本中极低水平...
TaqMan® Fast Advanced Master Mix has been validated for use with multiple real-time PCR applications, including microRNA assays. The formulation provides high specificity and dynamic range, the two most critical performance attributes that define successful results when working with microRNAs. The dat...
• The intersection of the threshold with the amplification plot defines the Ct in real-time PCR assays. The threshold is set above the background signal and within the exponential growth phase of the amplification curve. TaqMan™ SCID/SMA Plus Assay User Guide 17 A Safety WARNING! GENERAL...
TaqMan Advanced miRNA Assays with universal RT step provide highly sensitive and specific qPCR detection of mature miRNAs from serum and other sample types.
目前,最常用的检测和定量MicroRNA的方法是northern杂交, 芯片(microarrays)以及核糖核酸酶保护分析(ribonuclease protection assays),这些传统方法都需要标记探针并与纯化的RNA进行杂交。成熟的活性miRNAs及其前体共享一段相同的靶序列,而基于杂交的各种技术又无法通过分子大小将它们区分,因此很难专一性的识别成熟miRNAs,结果...
Comparison of singleplex and multiplex gene expression assays. (A) EGFR assay, FAM dye; (B) BRCA1 assay, VIC dye; (C) ESR1 assay, JUN dye; (D) RNase P assay, ABY dye. Amplification was performed on the QuantStudio 7 Real-Time PCR System using TaqMan Multiplex Master Mix. The figure...
TaqMan(R) Protein Assays (TPA) uses a noveltechnology called proximity ligation combined with qPCR for the detection and quantification of proteins in a homogeneousformat. This technique has been optimiz...