Why TaqMan Assay Design is Important Efficiency of Real-Time PCR What is multiplexing? Taq Talk Video Series Advantages of multiplexing Duplexing Three or more genes? Factors that affect the reliability of multiplex PCR assays General considerations Primer limitation V...
Confidence in your results is vital when people are counting on you. Detect your target 100% of the time with TaqMan® Assays and reagents. The combination of the most advanced assay design pipeline in the industry and the benefits of MGB probes delivers the specificity you need to detect ...
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LAMP assay is a relatively new DNA amplification technique, which due to its simplicity, ruggedness, and low cost could provide major advantages. LAMP has the potential to be used as a simple screening assay in the field or at the point of care by clinicians. Because Magigen LAMP assay is ...
This technology, which is highly sensitive, is commercially available as the TaqMan® (Applied Biosystems; Perkin-Elmer Corp.) and LightCycler® (Roche Diagnostics Corp.) systems. The advantages of real-time PCR are the rapidity of the assay (30–40 min), the ability to quantify and ...
The developed assay was compared with the gold-standard assay—TaqMan ACTB Gene Expression Assay (assay ID: Hs01060665_g1) (Thermo Fisher Scientific, Waltham, MA, USA)—performed with TaqMan Fast Advanced Master Mix (ThermoFisher Scientific, Waltham, MA, USA). The comparison was conducted on ...
The assessment and control of microbial contamination in health care facilities is presently a mandatory and vital part of strategies to prevent and control hospital-acquired infections. This study aims to assess the bioburden with two passive sampling methods (30 ventilations grids swabs and 16 elect...
[31] characterized the fanl locus in a soybean line A5 and developed a TaqMan assay for a complex insertion and deletion in the FAD3A gene. Development of SNP Genotyping Assays for Seed Composition Traits in Soybean Professor Festus Aghagbo Nwako Library (FANL), Nnamdi Azikiwe University Libra...
Broadly reactive TaqMan((R)) assay for real-time RT-PCR detection of rotavirus in clinical and environmental samples Rotaviruses are enteric pathogens responsible for a significant burden of disease, especially in children, through person-to-person transmission and exposu... N Jothikumar,G Kang,VR...
In May 2016, the World Health Organization adopted the first global hepatitis strategy, setting the ambitious goal of “elimination of viral hepatitis as a public health threat” by 2030. HCV-specific targets included a 65% reduction in HCV-related...