Thus it is necessary to validate the multiplex reaction thoroughly following the same guidelines outlined above for performing a duplex reaction. In a multiplex reaction with 2 or more targets, you can utilize TaqMan assays for the genes of interest, with each assay probe labeled with a different...
LAMP assay is a relatively new DNA amplification technique, which due to its simplicity, ruggedness, and low cost could provide major advantages. LAMP has the potential to be used as a simple screening assay in the field or at the point of care by clinicians. Because Magigen LAMP assay is ...
TaqMan SNP genotyping assays can be applied not only to animal species, but also to plant species that carry at least two paired sets of chromosomes. The 30–80% of plant species that are polyploid can also be genotyped using TaqMan Assa...
9 RegisterLog in Sign up with one click: Facebook Twitter Google Share on Facebook AcronymDefinition TAGLThere's a Good Lass TAGLToronto Area Gays & Lesbians(Canada) TAGLThere's a Good Lad TAGLTower Australia Group Limited(financial services company) ...
Stewart D, Zahiri R, Djoumad A, Freschi L, Lamarche J, Holden D et al (2016) A multi-species taqMan PCR assay for the identification of Asian gypsy moths (Lymantria spp.) and other invasive lymantriines of biosecurity concern to North America. PLoS ONE 11(8):e0160878. https://doi...
This technology, which is highly sensitive, is commercially available as the TaqMan® (Applied Biosystems; Perkin-Elmer Corp.) and LightCycler® (Roche Diagnostics Corp.) systems. The advantages of real-time PCR are the rapidity of the assay (30–40 min), the ability to quantify and ...
The assessment and control of microbial contamination in health care facilities is presently a mandatory and vital part of strategies to prevent and control hospital-acquired infections. This study aims to assess the bioburden with two passive sampling methods (30 ventilations grids swabs and 16 elect...
(manual and physical) were necessary significantly more often in GG (rs1800814) and CC (rs2070958) homozygotes. Conclusions: ThePDGFAgene’s polymorphisms influences the effectiveness of PRP therapy in tennis elbow treatment. The effectiveness of PRP is greater in A allele (rs1800814) and T ...
[31] characterized the fanl locus in a soybean line A5 and developed a TaqMan assay for a complex insertion and deletion in the FAD3A gene. Development of SNP Genotyping Assays for Seed Composition Traits in Soybean Professor Festus Aghagbo Nwako Library (FANL), Nnamdi Azikiwe University Libra...
A total volume of 25 [micro]L was used; the volume included 12.5 [micro]L of TaqMan Universal MasterMix (Life Technologies, Carlsbad, CA, USA) and 5 [micro]L of template DNA for the Plasmodium genus and B2MG assay as well as 3 [micro]L of template DNA for HSV-1, HSV-2, EBV,...