205311凯杰Qiagen反转录试剂盒QuantiTect RevTranscription Kit 品牌凯杰Qiagen 福州木辰生物科技有限公司 3年 查看详情 ￥4389.00/盒 上海 Qiagen逆转录试剂盒218161凯杰miScript II RT Kit 品牌qiagen 上海力敏实业有限公司 2年 查看详情 ￥3990.00/盒 江苏苏州 猪内源性逆转录病毒PCR检测试剂盒优博森生物订制...
In vitro transcription cDNA synthesis kits Reverse transcriptases RACE kits Purified cDNA & genomic DNA Purified total RNA and mRNA PCR Most popular polymerases High-yield PCR High-fidelity PCR GC rich PCR Direct PCR PCR master mixes Cloning In-Fusion seamless cloning Competent...
V. Protocol: reverse transcription: 4 TAKARA BIO INC. PrimeScript TM RT reagent Kit (Perfect Real Time) #RR037A v.06 0 http://.takara-bio *3. When using a Gene Speci c Primer: Perform the reverse transcription at 42℃ for 5 minutes. ...
Using a straightforward RNA-isolation protocol, the CellAmp RT-PCR for Direct Prep Kit (Real Time) & Protein Analysis allows direct RNA template purification from animal cells for use in one- and two-step qRT-PCR. The kit enables RNA isolation from animal...
cDNASynthesisKitManual TAKARABIOINC.http://.takara-bio Cat.#630 v.0708 TableofContents .Description...2 2.KitComponents...
• Try increasing the annealing temperature by increments of 2°C, or change to a two-step PCR protocol. • When the template contains small amounts of a target DNA, nested PCR may be effective. For PrimeSTAR HS and PrimeSTAR Max DNA Polymerases: • Check the annealing time setting ...
The protocols used for differentiation towards hepatocytes were analyzed for the formation of embryonic endoderm cells after the “endoderm induction” step, cells cultured according to protocol a) (FIG. 11). At day 7 in the differentiation protocol, cells were harvested for RNA and analyzed by Re...
实验12 除去 RNA 中的基因组 DNA DNase I ( RNase Free ) TaKaRa Code : D 在微量离心管中配制下列反应液,全量 50 μl 。全 RNA 20 ～ 50 μg 10×DNase I Buffer 5 μl DNase I ( RNase-free , 5 U/μl ) 2 μl RNase Inhibitor ( 40 U/μl ) 0.5 μ DEPC H2O up to 50 μl ℃...
TaKaRa LA PCR Kit Ver.2 (Cat.#RR013A/B) 2. 5' end-phosphorylated RT-Primer (for reverse transcription) 3. 1st PCR Primer pair (for 1st PCR) 4. 2nd PCR Primer pair (for 2nd PCR) 5. Mineral Oil (if necessary) 6. Agarose gel ...
This new and improved oligo, combined with SMARTScribe Reverse Transcriptase, provides higher specificity and increased yields. The SMARTer Pico PCR cDNA Synthesis Kit allows you to synthesize high-quality cDNA for array probe generation, cDNA subtraction, "Virtual Northern" blots, or other applications...