In-Fusion克隆技术凭借插入的目的DNA片段与载体末端的15个同源碱基序列就可以完成目的基因的定向克隆,操作简单快速。In-Fusion HD采用5×预混合酶试剂,大大缩短了反应时间,让您轻松操作。 ■ 制品特点 1. 设计随心而动:在任意载体位点插入任意基因片段 2. 真实的表达:依据15 bp同源序列实现无缝克隆,消除冗余碱基 3...
In-Fusion 克隆技术指南 克隆产品选择指南 In-Fusion克隆FAQ In-Fusion克隆Tips In-Fusion 简易操作流程 In-Fusion 多片段克隆protocol In-Fusion 引物设计工具 ■ 相关视频 2分钟了解In-Fusion的操作流程 多才多艺的In-Fusion无缝克隆 2分钟看懂In-Fusion作用机制的与众不同 产品详情请点击:此...
Infusion Hd Cloning Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/infusion hd cloning kit/product/TaKaRa ...
采用In-Fusion基因克隆技术可将扩增获得的目的基因片段直接克隆至预线性化pUC19载体中,而无需经过限制性酶切处理。将重组质粒转化至Stellar感受态细胞,通过菌落PCR扩增靶标区域。分析PCR产物的DNA序列确认突变位点序列。 图1.实验流程 Step1 Guide-it Indel Identification Kit用于确认细胞群中经过基因编辑后所导入的序列...
Protective screens in common areas Room sanitization opt-out available Rooms sanitized between stays Safe dining setup Sanitized kitchen and tableware items Staff trained in safety protocol Temperature check for guests and staff Dining, drinking, and snacking Bar Breakfast [buffet] Coffee shop Kitchen Re...
We showed that pertussis toxoid-IsdB (Ptx-IsdB) but not diphtheria toxoid-IsdB (Dpth-IsdB) fusion proteins protected SA-exposed mice from SA challenge (Supplementary Fig. 7a, b ). Fig. 5: IFN-γ and IL-17A, independent of IL-10, confer IsdBCAF01 vaccine protection against SA in SA-...
Figure 1. In-Fusion Cloning protocol.The key to In-Fusion Cloning is 15 bp of homology between your insert(s) and linearized vector backbone. One product, multiple applications In-Fusion Cloning is beautifully versatile. With an easy-to-follow protocol that provides high efficiency, you can suc...
Staff trained in safety protocol Temperature check for guests and staff Dining, drinking, and snacking Bar Breakfast [buffet] Coffee shop Kitchen Restaurants Shared kitchen Vending machine Swimming & soaking Hot spring bath Services and conveniences ...
Z-Taq polymerase can amplify a 1-kb DNA fragment from genomic DNA in 20 minutes, using a 30-cycle, two-step amplification protocol. The improved processivity of the Z-Taq enzyme also enables the amplification of longer fragments than standard Taq DNA polymerase (up to 20 kb from lambda DNA...
When a polypeptide is produced by genetic engineering, a peptide chain that is irrelevant to the activity of the polypeptide of interest may be added at the amino terminus or the carboxyl terminus of the polypeptide. For example, a fusion polypeptide, in which a portion of an amino terminus ...