Overexpression of TAB2 or TAB3 induces punctate localization of ATG5 under the normal culture condition. Knockdown of TAB2 and TAB3 results in the decrease in endogenous protein level of p62/SQSTM1 under the normal culture condition, while overexpression of TAB2 results in the accumulation of ...
TAK1 activity is tightly regulated by multiple binding partners including TAB1, TAB2 and TAB3, as well as post-translational modifications such as phosphorylation, ubiquitination and methylation [17–20]. TAK1 activation correlates with phosphorylation primarily on T187 and also on T178, T184 and...
转录程序。TAK1的激活需要形成异三聚体复合物,包括TAK1‑TAB1‑TAB2或TAK1‑TAB1‑TAB3, 其中TAB1结合到TAK1的N端激酶结构域,而TAB2和TAB3结合到C端区域。此外,TAK1的多种 PTMs,如T184/187的磷酸化,已被报道对TAK1的激活至关重要,值得注意的是,越来越多的 证据证明TAK1是一种对不同类型刺激作...
Ubiquitination and phosphorylation of TAK1 and its binding partners (TAB1, TAB2, and TAB3) are key factors that affect activation of downstream molecules, such as NF-kB, JNK, and p38 MAPKs. Using a GCN5-specific inhibitor, we found that the binding of TAK1 with TAB1 was reduced. As ...
2 结果 2.1 TAK1与TAB1蛋白在食管癌组织中高表达 免疫组化实验表明,TAK1与TAB1蛋白均表达于细胞质,细胞核基本无表达(图1)。其中,TAK1在癌组织中的高表达率为65.5%(55/84),显著高于癌旁组织(25.0%,21/84;P < 0.01);而TAB1在癌组织的高表达率为52.4%(44/84),也明显高于癌旁组织(27.4%,23/84;P...
TAB1 constitutively binds and activates TAK1, whereas TAB2 or TAB3 bind TAK1 after stimulation26. The TAK1 complex is activated in response to proinflammatory stimuli via K63-linked polyubiquitination driven by the E2 ligase UBC13/ UEV1A and the RING finger E3 ligases TRAF2 or TRAF6. K63...
TAB1β (Transforming Growth Factor-β-activated Protein Kinase 1-binding Protein 1β), a Novel Splicing Variant of TAB1 That Interacts with p38α but Not TAK1 The mitogen-activated protein kinases (MAPKs) play an important role in a variety of biological processes. Activation of MAPKs is me...
It is universally known that TAK1 is activated by a component composed of TAK1-binding protein 2 (TAB2) and RIPK1 polyubiquitin chain. Then activated TAK1 further phosphorylates IKKα/ IKKβ/IKKγ complex, which degrades IκB protein, leading to NF-κB activation29,30. On the other hand...
TAB2 KO MEFs. We found that compared to that of WT MEFs, the phosphorylation of RIPK1 S321 was enhanced and sustained longer at later time-points in TAB2 KO MEFs when the signals in WT MEFs were already subsided (Fig.2c). Consistent with the prolonged activation of TAK1, TNFα ...