1) tac promoter tac启动子1. -10 consensus sequence (TATGTT) of mutational tac promoter was reverted to TATATT and TATGAT by PCR amplification method. 应用PCR技术将发生突变的tac启动子-10区(TATGTT)回复突变为TATATT和TATGAT,构建了2个重组质粒pPM1和pPM2。
6) tac promoter tac启动子 1. -10 consensus sequence (TATGTT) of mutational tac promoter was reverted to TATATT and TATGAT by PCR amplification method. 应用PCR技术将发生突变的tac启动子-10区(TATGTT)回复突变为TATATT和TATGAT,构建了2个重组质粒pPM1和pPM2。 2. The PCR fragments were cloned...
These hybrid promoters, tacI and tacII, were derived from sequences of the trp and the lac UV5 promoters. In the first hybrid promoter (tacI), the DNA upstream of position -20 with respect to the transcriptional start site was derived from the trp promoter. The DNA downstream of position...
80, 21-25) the spacing between the -35 and -10 consensus sequences is 16 base pairs. Between these two regions we inserted 1 or 2 base pairs to increase the distance to 17 base pairs (trc promoter) or 18 base pairs (tic promoter). The activities of the three promoters were compared...
(1992) " Tac promoter vectors incorporating the bacteriophage gene 10 translational enhancer sequence for improved expression of cloned genes in Escherichia coli. " J Biotechnol. 23: 153-165.Lehmeier B, Amann E (1992) Tac promoter vectors incorporating the bacteriophage T7 gene 10 translational ...
the cells were co-transfected with the pGL3-E2F1 gene promoter luciferase or the pRL-TK Renilla luciferase construct, and 36 h later, the luciferase activity of theE2F1promoter was measured and the fold enrichment relative to the control siRNA was determined. Data are presented as mean ±...
权威例句 The tac promoter: a functional hybrid derived from the trp and lac promoters. Chimeric immunoglobulins specific for p55 TAC protein of the IL-2 receptor Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli ...
Transcribe a sequence of DNA to the complementary RNA sequence. What would the sequence of bases of the anticodons on the tRNA be? Fill in the blank: Any three base sequence found on the mRNA that codes for a specific amino ...
shRNA is used to knockdown the expression of TACC3 or KIF11 in Panc-1 cells. The sequences for shRNA are below: sh-Scr: CCTAAGGTTAAGTCGCCCTCG; shTACC3#1: GCTTGTGGAGTTCGATTTCTT; shTACC3#2: GTTACCGGAAGATCGTCTGT; TACC3-3’UTR: CCACGGAGCCGCTGTCCCCGC; ...
Codon is a nucleotide sequence made of three bases. These sequences represent a specific amino acid. There are four nucleic acid bases namely adenine, guanine, cytosine, and thymine (in DNA) or uracil (in RNA). And they can exist in 64 sequences...