(1992) " Tac promoter vectors incorporating the bacteriophage gene 10 translational enhancer sequence for improved expression of cloned genes in Escherichia coli. " J Biotechnol. 23: 153-165.Lehmeier B, Amann E (1992) Tac promoter vectors incorporating the bacteriophage T7 gene 10 translational ...
6) tac promoter tac启动子 1. -10 consensus sequence (TATGTT) of mutational tac promoter was reverted to TATATT and TATGAT by PCR amplification method. 应用PCR技术将发生突变的tac启动子-10区(TATGTT)回复突变为TATATT和TATGAT,构建了2个重组质粒pPM1和pPM2。 2. The PCR fragments were cloned...
The MCS in pEGFP-N1 is between the immediate early promoter of CMV (PCMV IE) and the EGFP coding sequences. Genes cloned into the MCS will be expressed as fusions to the N-terminus of EGFP if they are in the same reading frame as EGFP and there are no intervening stop codons. SV40 ...
After successful ligation, the plasmids were linearized for transcription, and recombinant RNA transcripts were then synthesized using the SP6 promoter and transfected into BHK cells (Supplementary Fig. 2). Virus preparation and neuron infection Sindbis virus was prepared as described previously [27,28,...
4O). Furthermore, ChIP assay of FOXM1 showed that FOXM1 binds to the promoter regions of TACC3 and KIFC1 (in addition to its known targets, CCNB1 and PLK1 [33, 34]) in MCF-7 p53−/− cells stronger than in p53-wt MCF-7 cells (Fig. 4P, Q). Overall, p53 loss/mutation ...
Constitutive expression of this gene under the control of the cytomegalovirus (CMV) promoter in mouse fibroblasts, results in cellular transformation and anchorage independent growth, suggesting that inappropriate expression can impart a proliferative advantage. This observation raises the possibility that ...
Results TERT promoter mutation was recorded in all GB and 6/14 LGG. Among the 7 cases with a methylation score > 0.9 in the classifier (v12.5), 2 were classified as glioblastoma, 4 as ganglioglioma (GG) and 1 as dysembryoplas‑ tic neuroepithelial tumor (DNET). t-SNE ...
promoter and the sacB gene. To create a unique HindIII cloning site in the vector, the HindIII sites of the P1 plasmid replicon (two sites), the P1 lytic replicon (one site), and the pRiA4 replicon (one site) were destroyed sequentially after ...
Cauliflower Mosaic Virus 35S promoter (vector sequence and map provided in DatafileS1). Plum transformation PpeTAC1-HGand35S::PpeTAC1vectors were transformed into theAgrobacterium tumefaciensstrain GV3101. Agrobacterium-mediated transformation of plum hypocotyl slices was performed as previously described...
shRNA is used to knockdown the expression of TACC3 or KIF11 in Panc-1 cells. The sequences for shRNA are below: sh-Scr: CCTAAGGTTAAGTCGCCCTCG; shTACC3#1: GCTTGTGGAGTTCGATTTCTT; shTACC3#2: GTTACCGGAAGATCGTCTGT; TACC3-3’UTR: CCACGGAGCCGCTGTCCCCGC; ...