3. Holton TA, Graham MW. A simple and efficient method for direct cloning of PCR products using ddT-tailed vectors. Nucleic Acids Res. 1991 Mar 11;19(5):1156 4. Marchuk D, Drumm M, Saulino A, Collins FS. Construction of T-vectors, a rapid and general system for direct cloning of...
Molecular cloning(3rdedition) 2.Shuman, S. (1991). Recombination Mediated by Vaccinia Virus DNA Topoisomerase I in Escherichia coli is Sequence Specific. Proc. Natl. Acad. Sci. 3.Holton TA, Graham MW.A simple and efficient method for direct cloning of PCR products using ddT-tailed vectors....
TA克隆技术
Directional cloning is made possible by appropriate hemi-phosphorylation of both the T-vectors and the inserts. With a single T-vector at hand, any DNA fragment can be cloned without compromising the cloning efficiency. The universal TA cloning method is thus both convenient and labor-saving....
4. ^ "TA Cloning Kit Manual". Invitrogen. 7 April 2004. pp. 31. http://stanxterm.aecom.yu.edu/wiki/data/Product_manuals_attach/ta.pdf. Retrieved 2009-10-17. 5. ^ Holton, T.A.; Graham, M.W (1991-03-11). "A simple and efficient method for direct cloning of PCR products using...
Cloning method: TOPO® TA Cloning®—Topoisomerase I–based, 5-minute ligation of PCR products with 3´A overhangs (Taq-amplified) to the vector Competent cells: Various options—choose from kits with general, high-efficiency, bacteriophage T1-resistant, or fast-growing competent cells, or ...
TA Cloning: - This cloning method was designed for use with pure Taq polymerases (native, recombinant, hot start); however, High Fidelity or Taq blends generally work well with TA cloning. A 10:1 or 15:1 ratio of Taq to proofreader polymerase will still generate enough 3' A overhangs ...
PCR Cloning by Incorporation of Restriction Sites The PCR cloning method we will employ involves engineering restriction sites into the PCR primers, so that those restriction sites will be incorporated into the PCR product to be cloned.Figure 3.2depicts a PCR reaction where restriction sites are eng...
Cloning method: TOPO™ TA Cloning™—Topoisomerase I–based, 5-minute ligation of PCR products with 3´A overhangs (Taq-amplified) to the vector Competent cells: Various options—choose from kits with general, high-efficiency, bacteriophage T1-resistant, or fast-growing competent cells, or ...
2. Objective To discuss the cloning method for detecting the HPRT gene mutation of rats. 目的 探讨克隆法检测大鼠体细胞HPRT基因突变 ,为后续研究打下基础。 更多例句>> 补充资料:分子克隆法 分子式:分子量:CAS号:性质:见基因工程。 说明:补充资料仅用于学习参考,请勿用于其它任何用途。参考...