An overview of Thermo Fisher Scientific's Gateway Cloning technique and how to simulate the Gateway reaction in SnapGene.
Therefore, we developed a Gateway cloning method that uses fusion-PCR to generate attL recombination site adaptors, and the PCR products, which can be directly cloned into destination vectors, giving rise to Rapid One-Step Gateway (ROG) Cloning. 100% of cloning efficiencies were obtained by ...
Enter the Gateway platform via Invitrogen TOPO cloning vectors containing Gatewayattsites or purchase an Thermo Scientific Ultimate ORF Clone already inserted into a Gateway vector New advancements such asMultiSite Gateway Technologymake Gateway cloning the ideal cloning method for protein expression and fu...
from the Lawson Lab website 1Gateway cloningrecombination-based cloning methodcomprised of two separate reactionsORFattB1 attB2ccdB/cam attP..
The Gateway cloning method, developed by Invitrogen in the 1990s, uses the site-specific recombination machinery of phage lambda to integrate its genome into E. Coli. LENGTH 4 Minutes A Detailed Look at Gateway Cloning INSIDE THE VIDEO Take a detailed look at ThermoFisher's proprietary system Ga...
Gateway 克隆技术背景原理及其应用
Gateway® 克隆技术背景、 原理及其应用 王宇、余辉 首都师范大学 Table of Contents 1 Overview 2 Advahtages of the Gateway Technology 3 Application of Gateway Technology 4 Examples What is “Gateway Technology”? ‘The Gateway® Technology is a universal cloning method based on the site-specific ...
ccdbecombigat克隆技术recombirecombinat Gateway®王宇、余辉首都师范大学TableofContentsOverview1AdvahtagesoftheGatewayTechnology2ApplicationofGatewayTechnology3Examples4Whatis“GatewayTechnology”?‘TheGateway®Technologyisauniversalcloningmethodbasedonthesite-specificrecombinationpropertiesofbacteriophagelambda.’Gateway...
在线询价 优惠:请询价 订货号:2XS5159 品牌型号:Invitrogen 12537100 货期:询货期 最小订货量:1套 +- Invitrogen 联系我们 400-920-3909 微信扫一扫,手机采购 产品介绍Product Description 规格 Cloning Method:Gateway® Key Functions:Clone your own Promoter, Multi-Gene Expression ...
The cloning vector pDown-Hiwi-3 × flag-IRES-hrGFP and expression vector pAV. Des1d were used for homologous recombination reaction to obtain recombinant adenovirus vector pAV.Ex1d-Hiwi-3× flag-IRES-hrGFP.The positive clones were selected by PCR to extract the recombinant adenovirus plas...