pBM28 Toposmart Cloning Kit(gateway系统入门TOPO克隆载体) 20次 ×3 BJ-PJ6528 产品介绍: 保存条件:-20℃ 保存 产品介绍: 利用痘苗病毒的拓扑异构酶I(Topoisomerase I)的切割再连接的特点将PCR产物定向克 隆到线性化的pBM28载体中。适用于克隆由Pfu、sPfu、KOD、Xerox、Phusion和Q5 等高 ...
英文名:5 minute directed Gateway entry cloning Kit 产地:国产|进口 产品介绍: 本产品采用了世界*进的Directional Topoisomerase Cloning(定向拓扑异构酶克隆技术)可以在5分钟内将待表达目的基因(高保真酶扩增的平末端片段)一步法定向克隆到Gateway入门载体(entry vector),得到的入门克隆(entry clone)可以和各种Gateway...
Gateway LR Cloning Kit20 ReactionsE084￥1150.00 ProClone™ Competent Cells5ml (4 x 1.25ml)E003$120.00 Column-Pure Plasmid Mini-Prep Kit100 PreparationsD504$85.00 产品留言 标题 联系人 联系电话 内容 验证码 点击换一张 注:1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
Summary of the Golden GATEway cloning kit.Stephan KirchmaierKatharina LustJoachim Wittbrodt
Directional TOPO Cloning Vector includes the Shine-Dalgarno sequence for E. coli expression-ready Entry clone pENTR/TEV/D-TOPO Cloning Kit K252520 Directional TOPO Cloning 5’ TEV sequence for N-terminal tag removal VERY GOOD High efficiency TOPO TA Cloning Vector Kits pCR8/GW/TOPO TA Cloning ...
Directional TOPO Cloning 5’ TEV sequence for N-terminal tag removal Includes fast-growing competent E. coli that shortens the time for miniprep pENTR/D-TOPO Cloning Kit K240020 Directional TOPO Cloning pENTR/SD/D-TOPO Cloning Kit K242020 Directional TOPO Cloning Vector includes the Shine-Dalgarno...
Refer to Current Protocols in Molecular Biology (Ausubel et al., 1994) or Molecular Cloning: A Laboratory Manual (Sambrook et al., 1989) for detailed protocols. What You Should See If you digest genomic DNA from your transfectants with an appropriate restriction enzyme that cuts at a single ...
Interestingly, some of the clones encoding functional modules in our toolkit could be exchanged with those from similar platforms built with the same MultiSite Gateway cloning kit as ours, expanding the number of possible fusion protein combinations. One example is the platform known as Tol2Kit [24...
2)常规DNA测序操作程序.常规质粒DNA测序程序按照BigDyeTerminatorv3.1SequencingKit 说明书进行.在10的反应体系中,分别加入1 质粒DNA(100ng),1.75L5×测序缓冲液,0.5L TerminatorReadyReaction混合液,0.15I10 tmaol/L测序引物(pHellsgate2F:TGACATCTC— CACTGAOGTAAG),6.6LddH2O.i 贝『JJ 芋PCR 反应 在GeneAmp...
pBM26 Toposmart Cloning Kit(gateway系统入门 TOPO克隆载体) 价格￥1060.8 起批量≥1盒 最小起订1盒 供货总量20盒 发货地址上海市青浦区 建议售价￥1060.8/盒 更新日期2025年01月09日 产品规格20 次 即时洽谈立即询价查看联系方式 收藏产品发送留言