By a map-based cloning method, the mutation of ygl8 was shown to be located in a 54-kb genomic region between InDel marker ID-3 and SSR marker RM12339 on chromosome 1. Furthermore, a nucleotide mutation from C to T in Loc_Os01g73450 was identified. By transgenic complementation and ...
Map-based cloning has been widely used to isolate and characterize many important plant resistance genes. However,most mapping strategies based on different molecular markers are very time-consuming and then make gene cloning difficult.Here we present a useful method for rapidly cloning rice resistance...
Map-based cloning of the pear gene MYB114 identifies an interaction with other transcription factors to coordinately regulate fruit anthocyanin biosynthesis... - 《Plant Journal》 被引量: 0发表: 2017年 Integrin signalling regulates YAP and TAZ to control skin homeostasis The skin is a squamous epit...
Map-based cloning and characterization of BPH29, a B3 domain-containing recessive gene conferring brown planthopper resistance in rice. J. Exp. Bot. 66, 6035–6045 (2015). Article CAS PubMed PubMed Central Google Scholar Huang, X. et al. High-throughput genotyping by whole-genome ...
Linkage maps are important tools for map-based cloning, marker-assisted breeding, QTL identification, genome organization, and comparative genomics of important species. A number of linkage maps have been constructed for several grasses including pearl millet39. However, so far, napiergrass SSR marker...
These mapped contigs not only organize a framework to study the Aquilegia genome, but also pave the way for gene isolation and characterization by map-based cloning approach to further understand the genes of interest. The genes involved in anthocyanin pigmentation biosynthesis in wheat are arranged ...
Map-based cloning has always been a long and tedious process. The genetic-physical map provides a shortcut by which contigs spanning a target gene region can be readily identified through flanking markers. Markers immediately upstream and downstream of a target gene can be used to identify neighbo...
Additionally, a higher average coverage of one marker per 0.37 cM achievable by GBS would reduce the chance of missing any double-crossovers in the mapping population that were not identified by a PCR-based method [18]. Based on the GBS results we obtained with Illumina short-read sequencing ...
The work will not only help to understand the mechanisms that control yield traits of maize, but also provide a basis for marker-assisted selection and map-based cloning in further studies. 展开 关键词: bin map genotyping by sequencing maize quantitative trait loci yield ...
Mapping of intra-locus duplications and introgressed DNA: Aids to map-based cloning of genes from complex genomes illustrated by physical analysis of the R... We describe here novel approaches to high-resolution mapping of repeated and introgressed DNA which may prove generally useful in map-based...