A major quantitative trait locus,Hd1, that controls response to photoperiod was identified by the use of a map-based cloning strategy. High-resolution mapping using 1,505 segregants permitted the definition of a genomic region of about 12 kb as a candidate forHd1. Further analysis revealed ...
1.This method thus has been successfully applied toperform positional cloning and to construct detailed transcription maps.该法已成功地应用于定位克隆和详尽的转录图谱的构建。 2.One strategy of locatingtumor suppressor genes and cloning these genes is to analyse the high rates of LOH in humanmalignant...
These parents are also used for establishing populations for map-based cloning. Five BAC and TAC libraries were constructed. The general strategy for map-based cloning in L japonicus is described and map positions of eleven sym genes are shown. Four L japonicus sym genes have been isolated ...
The broad-spectrum rice blast resistance gene Pi9 was cloned using a map-based cloning strategy. Sequencing of a 76-kb bacterial artificial chromosome (BAC... Qu,S. - 《Genetics》 被引量: 1200发表: 2006年 Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled...
We report the map-based cloning of the leaf rust resistance gene Lr21, previously mapped to a gene-rich region at the distal end of chromosome arm 1DS of bread wheat (Triticum aestivum L.). Molecular cloning of Lr21 was facilitated by diploid/polyploid shuttle mapping strategy. Cloning of ...
The breeding of host-resistant crops is an economical and environmentally friendly strategy for pest control, but few resistance gene resources have thus far been cloned. An indica rice introgression line RBPH54, derived from wild rice Oryza rufipogon, has been identified with sustainable resistance ...
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Map-based cloning revealed BhAPRR2, encoding a two-component response-regulating protein that regulates the black peel formation of mature fruit in wax gourd. Abstract Wax gourd is an economically significant vegetable crop, and peel color is a crucial agronomic trait that influences its commercial ...
These robust resources will be useful for trait mapping, genetic diversity and linkage disequilibrium studies or map-based cloning, and will enable faba bean MAS and GAB as well as the identification of candidate genes of agronomic interest through synteny-based approaches....
Therefore, the fine mapping of these QTL and validation of the potential candidate genes may be a reliable and feasible strategy for QTL cloning to isolate loci that may be even more valuable for maize breeding. Our results provide important information for such further fine mapping to find ...