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Synthetic DNA Oligonucleotides Synthetic dNa oligonucleotides, which are either entirely double-stranded or mostly single-stranded, with a double-stranded T7 promoter sequence can be used in the T7 Quick transcription reaction. In general, the yields are relatively 4 low and also variable depending ...
The CLuc AG control template is a linearized plasmid containing theCypridina luciferasegene under the transcriptional control of the T7 promoter. The initiating sequence has been changed to an AG by site-directed mutagenesis to be compatible with CleanCap Reagent AG. The size of the run-off trans...
T7 Promoter Start of Transcription T7 Transcription Run-off transcript has the top strand sequence. (DNA template) (RNA transcript) 5´-TAATACGACTCACTATAGGG 3´-ATTATGCTGAGTGATATCCC 3´ 5´ 5´-GGG 3´ 4 P asmid Temp ates It is f the utm st im t nce t begin the HiS...
vitro transcription with the HiScribe T7 Quick High Yield RNA Synthesis Kit, provided that they contain a double-stranded T7 promoter region upstream of the sequence to be transcribed. Figure 1 illustrates the minimal T7 promoter sequence, as well as a run-off transcript after T7 transcription...
Description:Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase (99 kDa) that catalyzes in vitro RNA synthesis from a DNA sequence containing a T7 phage promoter.The highly concentrated version T7 RNA Polymerase HC is ideally suited for set-up and optimization of high yield in ...
Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase (99 kDa) that catalyzes in vitro RNA synthesis from a DNA sequence containing a T7 phage promoter. The highly concentrated version T7 RNA Polymerase HC is ideally suited for set-up and optimization of high yield in vitro transc...
By using a DNA template with a T7 promoter sequence followed by an AG initiation sequence and an encoded poly(A) tail, mRNAs can be transcribed with a 5´-m7G Cap-1 structure that is polyadenylated, translationally competent and able to evade the cellular innate immune response. The ...
1, we did not know which promoter (if any) will outperform the WT, so the name T7Max was not yet assigned to any sequence. For clarity, to avoid having the same sequence under two different names, we use label T7Max on all figures. Next, we proceeded to test full translation ...
oligonucleotides canbe used as templates for in vitro transcription with the HiScribe T7 Quick High YieldRNA Synthesis Kit, provided that they contain a double-stranded T7 promoterregion upstream of the sequence to be transcribed. Figure 1 illustrates theminimal T7 promoter sequence, as well as ...