promotergeneoverexpressionhighlevelsBecause various genome projects have been advanced many genes are known, and large amounts of proteins are required to elucidate their function. Most biomolecular research laboratories have a need to overexpress a certain gene, or a part of it, in eukaryotic or ...
To enhance the efficiency of the expression of target gene in eukaryotic cells, one of the strongest prokaryotic expression systems, the T7 RNA polymerase and T7 promoter, was introduced into eukaryotic cells. A duel-plasmid gene expression system of T7 bacteriophage components was developed; one...
normallylac. As a result, expression is switched on by the addition of lactose or IPTG, which enables the expression of the RNA polymerase that, in turn, recognizes the T7 promoter and enables expression of the heterologous protein. Expression vectors using this technology are termed pET plasmids...
Gene expression using the vaccinia virus/T7 RNA polymerase hybrid system - Elroy-Stein, Moss - 1991 () Citation Context ...has become a standard system for assaying mammalian PGPs, as it provides efficient heterologous expression of functional eukaryotic plasma membrane proteins and suppression of ...
Maximum Consistency and Minimum Variability The T7-Oligo(dT) Promoter Primer Kit contains: — T7-Oligo(dT) promoter primer (50 µM) — RNase-free water This reagent kit is an essential component of the eukaryotic target labeling procedure for expression analysis using GeneChip® probe arrays...
KeywordsT7RNApolymerase,T7promoter,prokaryoticexpressionsystem,eukaryoticcell 基于T7噬菌体RNA聚合酶(T7RNAP)及其启 动子之间识别的特异性和高效性而建立的表达系 统,为目前在大肠杆菌中克隆和表达重组蛋白的最 强大表达系统之一 [1] 。其中,T7噬菌体启动子属于 ...
现货中心:此类产品将由您身边的现货中心极速配送。 This reagent kit is an essential component of the eukaryotic target labeling procedure for expression analysis using GeneChip™ probe arrays. It contains 50 μM T7-Oligo(dT) promoter primer and RNase-free water. ...
3 Conrad,B.,Savchenko,R.S.,Breves,R.&Hofemeister,J.A T7 promoter-specific,inducible protein expression system for Bacillus subtilis.Molecular&general genetics:MGG 250,230-236(1996). 4 Fuerst,T.R.,Niles,E.G.,Studier,F.W.&Moss,B.Eukaryotic transient-expression system based on recombinant ...
Key Words :T7 promoter ;eukaryotic expression system ; T7 RNA polymerase 中图分类号 :Q523 + . 8 文献标识码 :A 文章编号 :1004 - 311X(2000) 05 - 0003 - 04 兔防御素(MCP - 1) cDNA 的克隆与序列分析 3 刘万臣 1 ,张玉静 1 ,张艳孙 1 ,谭 芳 1 , 李红卫 1 ,欧阳红...
coli-Based Cell-Free Protein Synthesis: Pre-Expression Endotoxin Removal Approaches for on-Demand Cancer Therapeutic Production. Biotechnol. J. 14, 1800271 (2019). Article Google Scholar Nagappa, L. K. et al. A ubiquitous amino acid source for prokaryotic and eukaryotic cell-free transcription-...