可以特异性检测抗原特异性T细胞T-SelectMHCTetramer.PDF,可以特异性检测抗原特异性T细胞 T-Select MHC Tetramer 在 T 细胞上表达的 T 细胞受体(T Cell Receptor,TCR)可以特异性识别MHC 分子与肽片段的复合 T-Select MHC Tetramer 物(MHC/peptide complex)并与其结合。1
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Reagent for direct detection of antigen-specific T cells. MHC tetramer technology is based on the ability of MHC-peptide complexes to recognize the antigen-specific T cells at a single cell level.
实验数据表明,与多量体(Multimer)相比,MBL MHC四聚体(Tetramer)的非特异性染色较少,可得到清晰的染色成像。 MHC四聚体技术不仅使得抗原特异性T细胞变得可以直接检测,并且还可以用于分离抗原特异性T细胞。从而可以对抗原特异性T细胞的功能和表型进行详细的...
② 在检测试管中加入10µL T-Select MHC Tetramer。③ 在检测管中加入200µL全血，轻柔地涡旋混匀...
Figure 2.HLA-DR-specific memory B cell clones isolated from peripheral blood using ProT2® MHC Class II Tetramers. A, Representative example of 3 independent experiments depicting the flow cytometry gating strategy to live single cell sort CD3-CD27+IgD-HLA-DR tetramer double positive B cells ...
Direct visualization and quantification of antigen-specific T cells using major histocompatibility complex (MHC)/peptide tetramer technology offers a powerful means to study specific T cell populations of interest. In combination with functional assays, this technology has already provided many new insights...
MHC-class I tetramers technology enabled the characterization of peptide-specific T cells at the single cell level in a variety of studies. Several laboratories have also developed MHC-class II multimers to characterize Ag-specific CD4+ T cells. However, the generation and use of MHC-class II ...
ProImmune 是全球领先在于提供荧光标记的人类 MR1 四聚体。 MR1 复合体结合在 mucosal-associated invariant T (MAIT) 细胞的 T 细胞受体。MAIT 细胞的特殊性(确定在使用的相关 MR1 结合小分子配体上),允许鉴定,列举和隔离抗原特异性 MAIT 细胞在流式细胞术。
PEPTIDE–MHC TETRAMERS Recombinant MHC molecules can be engineered to contain the antigenic peptide of interest in their peptide-binding groove. Biotinylation, followed by incubation with fluorescently labelled streptavidin, generates tetrameric complexes. These can be used to stain T cells that express ...