MHC class-I tetramer stainings of HIV- and CMV-specific CD8+ T cells in chronic HIV infection.Marcus BuggertJohanna TauriainenTakuya YamamotoJuliet FrederiksenMartin A. IvarssonJakob MichaëlssonOle LundBo HejdemanMarianne JanssonAnders Sönnerborg...
四聚体组装:单体MHC class I/多肽复合物与荧光标记链霉亲和素结合组装,最终形成四聚体,从而完成MHC Tetramer的制备 产品数据 天然构象、高纯度 经SDS-PAGE和HPLC-MALS验证,ACROBiosystems开发的天然构象MHC-多肽复合物,纯度高于90%。 The purity of Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) ...
实验数据表明,与多量体(Multimer)相比,MBL MHC四聚体(Tetramer)的非特异性染色较少,可得到清晰的染色成像。 MHC四聚体技术不仅使得抗原特异性T细胞变得可以直接检测,并且还可以用于分离抗原特异性T细胞。从而可以对抗原特异性T细胞的功能和表型进行详细的...
Soluble tetramers of major histocompatibility complex (MHC) class I or class II molecules are a biotechnological reagent produced in the laboratory to analyze the specificity of antigen receptors expressed by T cells. In nature T cell receptors (TCR) recognize peptide antigens bound to membrane MHC...
MHC Tetramer Soluble tetramers of major histocompatibility complex (MHC) class I or class II molecules are a biotechnological reagent produced in the laboratory to analyze the specificity of antigen receptors expressed by T cells. In nature T cell receptors (TCR) recognize peptide antigens bound to...
Figure 2.HLA-DR-specific memory B cell clones isolated from peripheral blood using ProT2® MHC Class II Tetramers. A, Representative example of 3 independent experiments depicting the flow cytometry gating strategy to live single cell sort CD3-CD27+IgD-HLA-DR tetramer double positive B cells ...
Direct enumeration of Borrelia-reactive CD4 T cells ex vivo by using MHC class II tetramers We characterized antigen-specific CD4+T cells in six patients with treatment-resistant Lyme arthritis, using an HLA-DRB1*0401 major histocompatibility comp... AL Meyer,C Trollmo,F Crawford,... - 《Pro...
抗原特异性CD4+ T细胞的直接检测一直很困难,因为这些T细胞的数量少(低至100,000个白细胞中的1个)和亲和力低,ProT2® MHC Class II Tetramers四聚体允许研究者通过流式细胞术准确检测单个抗原特异性CD4+T细胞。它们也可以用来分离细胞,用于培养、扩增和进一步研究。ProT2® MHC Class II Tetramers研究者能够深入...
Major histocompatibility (MHC) class I tetramers are used in the quantitative analysis of epitope peptide-specific CD8+ T-cells. An MHC class I tetramer was composed of 4 MHC class I complexes and a fluorescently labeled streptavidin (SA) molecule. Each MHC class I complex consists of an MHC...
Class I custom Tetramer Human, Mouse, Monkey, Chicken Class II custom Tetramer Human MHC custom tetramer kits QuickSwitch™ Custom Tetramer Kits Kits for preparation of custom tetramers in the laboratory using our proprietary peptide exchange technology. ...