CFSE kit来自于thermo, 以下为实验步骤: CFSE assay For new CFSE tube, add 18 ul DMSO into the tube, to get 5 mM (working solution 1:1,000 dilute) 1 mL complete RPMI for 10e6 cell; 0.5 ml for < 5e6 cell 1.1 ul 5mM in 110 ul PBS In tube bottom, put 1ml cells In tube top, ...
(提示：染色后，确保避光操作)接着，执行T细胞活化步骤，使用thermo的CD3/CD28 dynabeads激活T细胞。最终结果如图所示，红色曲线代表对照组，绿色部分表示活化的T细胞。每次增殖后，CFSE荧光强度会减少一半。这就是CFSE T细胞增殖实验的全过程，是不是非常简便呢？
东大生物的方法可以评估测试品在由CD3 / CD28抗体诱导下的T细胞活化过程中的细胞增殖作用,测试方式包括CFSE标记稀释法和细胞内IFN-γ水平将通过流式细胞仪(FCM)测试法。 测试程序和方法 代表性结果 T cell response in mouse CD4+T and CD8+T cell activation/proliferation assay for 2 days as measured by CFSE...
Inhibition of T-cell proliferation is the most common approach to assess human myeloid-derived suppressor cell (MDSC) functions. However, diverse methodologies hinder the comparison of results obtained in different laboratories. In this chapter, we present a T-cell proliferation assay procedure based ...
Kurata Y, Kato M, Kuzuya T, Miwa Y, Iwasaki K, Haneda M, Amioka K, Watarai Y, Uchida K, Nakao A, Kobayashi T. Pretrans- plant pharmacodynamic analysis of immunosuppressive agents using CFSE-based T-cell proliferation assay. Clin. Pharmacol. Ther., 86, 285-289 (2009)....
Contact ProMab today to discuss T cell activation and proliferation assay services and more! Request A Quote Other Components of ProMab's Cell Based Assay Platform: Flow Cytometry Reference 2017Oct 17;47(4):621-634. doi: 10.1016/j.immuni.2017.09.008. Authors. Ira Tabas ,AndrewHLichtman ...
T cell proliferation assay A total of 5 × 106 T cells were loaded with carboxyfluorescein diacetate succinimidyl ester (CFSE) (Invitrogen; cat. no. C34554) according to the manufacturer’s instructions. Cells were blocked with 50% FBS, washed twice with RPMI and stimulated with 1 μg ...
With assistance of CFSE labeling assay, the flow cytometry is able to obtain moredetail information of proliferative response and dynamics of different T cell subpopulations after stimulated with different stimuli.[Key words] ? Carboxyfluorescein diacetate succinimidyl ester; Proliferation; T cell ...
CFDA SE Cell Proliferation Assay and Tracking Kit 产品规格:500T 1000T 保存条件:-20℃干燥避光保存,有效期 1 年 产品简介: CFDA, SE 细胞增殖与示踪检测试剂盒是基于 CFDA, SE 对细胞进行示踪及增殖检测的试剂盒,该试剂盒主要工作原理为:CFDA, SE 是一种膜通透性的荧光素染料,本身不具有荧光发光性...
流式细胞术检测CFSE标记人T细胞亚群增殖反应_王敏