CFSE kit来自于thermo, 以下为实验步骤: CFSE assay For new CFSE tube, add 18 ul DMSO into the tube, to get 5 mM (working solution 1:1,000 dilute) 1 mL complete RPMI for 10e6 cell; 0.5 ml for < 5e6 cell 1.1 ul 5mM in 110 ul PBS In tube bottom, put 1ml cells In tube top, ...
T细胞增殖测试(T Cell Proliferation Assay) T细胞的增殖测定试验可确定受试品对T细胞在细胞活化过程中促进细胞增殖的作用。东大生物的方法可以评估测试品在由CD3 / CD28抗体诱导下的T细胞活化过程中的细胞增殖作用,测试方式包括CFSE标记稀释法和细胞内IFN-γ水平将通过流式细胞仪(FCM)测试法。 测试程序和方法 代表性...
货号 名称 规格 价格(元) S4015 MTT Cell Proliferation Assay Kit (MTT细胞增殖及细胞毒性检测试剂盒 ) 500T 387 S4025 MTT Cell Proliferation Assay Kit (MTT细胞增殖及细胞毒性检测试剂盒 ) 1000T 682 产品简介
Cell samples were analyzed in triplicate in the IFN-gamma ELISPOT assay to determine their potential to respond to antigen stimulation. Results from test wells were compared to those for cells that were not stimulated (no peptide/negative control), and to those for cells stimulated with ...
Inhibition of T-cell proliferation is the most common approach to assess human myeloid-derived suppressor cell (MDSC) functions. However, diverse methodologies hinder the comparison of results obtained in different laboratories. In this chapter, we present a T-cell proliferation assay procedure based ...
Contact ProMab today to discuss T cell activation and proliferation assay services and more! Request A Quote Other Components of ProMab's Cell Based Assay Platform: Flow Cytometry Reference 2017Oct 17;47(4):621-634. doi: 10.1016/j.immuni.2017.09.008. Authors. Ira Tabas ,AndrewHLichtman ...
Alkan, S.S.: Assay for antigen-specific T-cell proliferation in mice. In I. Lefkovits and B. Pernis (eds.): Immunological Methods 1 , pp. 309–315, Academic Press, New York, 1979Alkan SS. Assay for antigen-specific T-cell proliferation in mice. In: Lefkovits I, Pernis B, eds....
CD3, CD4, CD8 T cell expansion Optimal bead:cell ratio T cell stimulation assay Expansion of human T cells. 视频 Unique Time-lapse Video of Dynabeads Activating T-Cells Get physiological activation and highly consistent activation, expansion using Dynabeads magnetic beads. Activate T-cells in vitro...
T cell proliferation assay To assess T cell proliferation, PBTs or naive T cells were stained with 1 µM 5-carboxyfluorescein diacetate (CFDA) (Thermo Fisher Scientific) for 20 min at 37 °C and 5% CO2 before they were cultured in serum-free medium (XVIVO-15, Lonza, Basel) with...
5b). The results of the proliferation assay showed CAR-T cells proliferated well in vitro (Supplementary Fig. 5c). The phenotype of CAR-T cells, including the ratio of CD4 and CD8 phenotypes and the ratio of different memory phenotypes, was detected by flow cytometry. The mean percentage of...