接着，执行T细胞活化步骤，使用thermo的CD3/CD28 dynabeads激活T细胞。最终结果如图所示，红色曲线代表对照组，绿色部分表示活化的T细胞。每次增殖后，CFSE荧光强度会减少一半。这就是CFSE T细胞增殖实验的全过程，是不是非常简便呢？
【工具】CFSE T cell proliferation assay 今天分享一个测试T细胞增殖的实验,可用于测试一些蛋白对于T细胞增殖的影响。 CFSE kit来自于thermo, 以下为实验步骤: CFSE assay For new CFSE tube, add 18 ul DMSO into the tube, to get 5 mM (working solution 1:1,000 dilute) 1 mL complete RPMI for 10e6 ...
文档标签: CFSE Cell Proliferation Assay - AAT BioCFSE细胞增殖试验- AAT生物 系统标签: cfse aat assay proliferation bio 增殖 AATBioquest®ProductTechnicalInformationSheetLastUpdatedFebruary2012©2012byAATBioquest®,Inc.,520MercuryDrive,Sunnyvale,CA94085.Tel:408-733-1055Ordering:sales@aatbio;Tel:800...
CFSE染色后细胞死太多? 做细胞增殖跟踪的同学,大多离不开CFSE。 但是,大多数人要么染不上,要么细胞死太多。这篇2011年的《Development of a flow cytometry assay combining cell proliferation, viability and two specific surface markers》,里面概括了不同浓度 ...
做细胞增殖跟踪的同学,大多离不开CFSE。 但是,大多数人要么染不上,要么细胞死太多。这篇2011年的《Development of a flow cytometry assay combining cell proliferation, viability and two specific surface markers》,里面概括了不同浓度 的CFSE培养MNC后,不同时间点的细胞死活情况,可以给我们一些启示。文中作者后来...
Protocol: Positive magnet isolation of CD4+ T cells from human PBMCs Poster: A Comparison of Three Techniques to Induce Efficient Ex Vivo T Cell Expansion Usage Example (Japanese): Analysis of mouse T cell...
Simple, robust staining protocol The CellTrace™ CFSE Cell Proliferation Kit contains convenient single-use vials of dry dye to permit small-scale experiments without preparing excess quantities of dye. A stock solution is prepared by dissolving the contents of a vial in anhydrous DMSO prior to ...
Here, we show a simple protocol to study human T and NK cell proliferation with CFSE dilution assay by flow cytometry.doi:10.1016/bs.mie.2019.05.020Iigo.TerrénBiocruces Bizkaia Health Research Institute, Immunopathology Group, Barakaldo, Spain.Ane.Orrantia...
This procedure has been optimized for labeling mouse and human lymphocytes at cell concentrations of 10-30 × 10^6 cells/mL. Assay conditions may need to be optimized for other cell types or cell densities. 1.Thaw the 10 mM stock solution of CFSE, if previously frozen. ...
CFSE-assay-lecturePPT课件 CelldivisiontrackingusingCFSE BruceLyons,DivisionofHaematology,IMVS,HansonInstitute,Adelaide - 1 Workshopoutline:-IntroductiontoCFSE.-Someofthecrucialthingsyouneedtoconsider.-Abasicprotocolaroundwhichtoplanyourapplication.-Coffeebreak?-Questiontime:*specificproblemsyoumayhavebeen...