The (2′–5′) A synthetase activity is induced in HeLa cells by IFN-α treatment and is activated after reovirus infection, since cell lysates from these cells synthesize in vitro (2′–5′) A oligonucleotides. The IFN-induced protein kinase activity is also triggered in those lysates upon...
Ambion. DNA oligonucleotides V1 (5 0 -CCATGTGCCAGGACAT- CAAAAGGG-3 0 ), IBArev (5 0 -CGCAGTAGCGGTAAACGGC-3 0 ), E32dir (5 0 -CTTGATATCCTGGCCGAAGCC-3 0 ) for site-directed mutagenesis, E7500 (5 0 -CACGCTCACCGGCTCCAGAT-3 ...
To construct the fungal gene expression vector pAB-nat, oligonucleotides MCS_pAB and MCS_pAB-rev (Table S1) were annealed, digested withNotI andBamHI, and ligated intoNotI andBamHI-digested plasmid pGG-C-EGFP (Table 2), thereby replacing theegfpandlacZgenes and inserting two newBsaI sites fo...