RNABase PairingBase SequenceDNA PrimersDeoxyribonucleotidesEvolution, MolecularNonenzymatic template-directed copying of sequences rich in cytidylic acid using 5'-(2-methylimidazol-1-yl phosphates) as substrates is substantially more efficient than the copying of corresponding DNA sequences. However, many...
A ribonuclease which degrades RNA specifically in RNA路DNA hybrid structures has been purified 1500-fold from B and 200-fold from strain D110 A, I, ). For maximal activity, the enzyme requires Mgand the presence of a sulfhydryl reagent. ... I Berkower,J Leis,J Hurwitz - 《Journal of ...
We herein demonstrate an innovative example of TGS using DNA nano-templates that promote Huisgen cycloaddition from an array of azide and alkyne fragments. A G-quadruplex and a control duplex DNA nano-template have been prepared by assembling the DNA structures on gold-coated magnetic nanoparticles....
RNA oligonucleotides have emerged as a powerful therapeutic modality to treat disease, yet current manufacturing methods may not be able to deliver on anticipated future demand. Here, we report the development and optimization of an aqueous-based, template-independent enzymatic RNA oligonucleotide synthes...
- 《Journal of Biological Chemistry》 被引量: 218发表: 1984年 RNA priming coupled with DNA synthesis on natural template by calf thymus DNA polymerase... Presents a survey on a bovine genomic DNA library with respect to the template activity for RNA-primed DNA synthesis by calf thymus DNA ...
A method is described to synthesize small RNAs of defined length and sequence using T7 RNA polymerase and templates of synthetic DNA which contain the T7 promoter. Partially single stranded templates which are base paired only in the -17 to +1 promoter region are just as active in transcriptio...
This chapter presents a method that provides a simple approach for the synthesis of a DNA template, containing RNA polymerase promoter sites, by using the polymerase chain reaction (PCR). A general schemata for the synthesis of a templat... Leonard,Sherry 被引量: 0发表: 1995年 Optimization of...
如何鉴别Identification of RNA polymerases的活性?可以通过将细胞提取物与NTPs共孵育的方法。 病毒粒子中RNA存在形式 Universal rules for RNA-directed RNA synthesis · RNA synthesis initiates and terminates at specific sites on the template · RdRp may initiate synthesis de novo (like cellular DdRp) or re...
Besides the activities of template-engaged RNA polymerases, a pool of "free" RNA polymerases is found, presumably not engaged in RNA synthesis in vivo. This pool decreases from high values in the early blastoderm stage to low levels during middle and late blastoderm stages and rises again to...
Using a 40 base oligonucleotide template containing the sequence of the preferred priming site, primase synthesizes RNA primers of 9 and 10 nt in length with the sequence 5'-(G)GAAGAAAGC-3'. These studies demonstrate that pol alpha-primase selects specific nucleotide sequences for RNA primer ...