template RNA- the template for protein synthesis; the form of RNA that carries information from DNA in the nucleus to the ribosome sites of protein synthesis in the cell informational RNA,messenger RNA,mRNA ribonucleic acid,RNA- (biochemistry) a long linear polymer of nucleotides found in the nu...
The nucleocapsid protein (NP) of Sendai virus encapsidates the genome RNA, forming a helical nucleocapsid which is the template for RNA synthesis by the viral RNA polymerase. The NP protein is thought to have both structural and functional roles, since it is an essential component of the NP0...
Methods of adding nucleotides to the double stranded template/primer substrate are also described. The method can be used to facilitate detection, PCR amplification, cloning, and determination of RNA and DNA sequences.ランボウィッツ, アラン エム.モア, サビーン...
Chemical modification of DNA is a common strategy to improve the properties of oligonucleotides, particularly for therapeutics and nanotechnology. Existing synthetic methods essentially rely on phosphoramidite chemistry or the polymerization of nucleosid
摘要: In the presence of σ, E. coli RNA polymerase forms rifampicin resistant complexes with DNA in the absence of nucleoside triphosphates. These complexes only form at promoter sites, so the number of σ-recognized promoters per genome for several DNA phages could be determined....
M. et al. Cap 1 messenger RNA synthesis with co-transcriptional CleanCap® analog by in vitro transcription. Curr. Protoc. 1, e39 (2021). CAS PubMed Google Scholar Grier, A. E. et al. pEVL: a linear plasmid for generating mRNA IVT templates with extended encoded poly(A) sequences...
The 550-bp fragment at the 30-end of GhAPm cDNA was used as the template for probe synthesis. There were no restriction sites for EcoRI and HindIII present in the probe region (Fig. 1). The result of hybridization indicated 123 Mol Biol Rep Fig. 5 Phylogenetic tree of GhAPm and ...
For SNAI1 knockdown, the pSLenti-U6-CMV-EGFP-F2A-Puro-WPRE vector was employed to construct short hairpin RNA (shRNA) plasmids. For PIK3R2 knockdown, the pSLenti-U6-CMV-EGFP-F2A-Puro-WPRE vector was utilized to construct short hairpin RNA (shRNA) plasmids. All these plasmids were ...
S1). For comparative purpose, we generated two model RNAs with either a single CMP (Template “C”) or a single NHC-MP (Template “N”) at position 11. To test whether an incorporated NHC-MP still acts as a C-analogue when present in the template we monitored RNA synthesis in the ...
The plastid-encoded RNA polymerase serves as the principal transcription machinery within chloroplasts, transcribing over 80% of all primary plastid transcripts. This polymerase consists of a prokaryotic-like core enzyme known as the plastid-encoded RNA