process for preparation of a double stranded dna in a single stranded dna template.the method consists of: a) a first strand of dna; and (b) adding a tail at the 3 '- end cell, the first strand of dna to produce a dna strand to the first line; and (c), a single stranded ...
The direction synthesisof biofunctional nanomaterials with DNAas the template is of high application value. By using phosphorothioate–thyminesingle-stranded DNA (PS–T–ssDNA) as the template andthrough synthetic conditions optimization... R Brijder,L Qian 被引量: 0发表: 2017年 ATP-Dependent Allost...
The DNA template activity of older cells was shown to be significantly lower on a per cell basis than that of younger cells. The majority of this reduction was shown to be due to the gradual loss of DNA template with an increase in fission age. The specific activity of the DNA template,...
sites deduced from the original sequence. Subsequently, 16 degenerate oligonucleotides were designed corresponding to the eight amino acids, and used to screen in the second round of DNA shuffling. Another mutated sequence was identified exhibiting a lower specific activity (Xiong et al., 2007a). ...
Schematic illustration of BCW synthesis. a Sequential HCR and PC on the live cell. HCR: hybridization chain reaction. PC: polyelectrolyte complexation. b Formation of the supramolecular DNA template and the concomitant DNA-templated alginate assembly during HCR. Cholesterol-conjugated DNA initiators (i...
Chemical modification of DNA is a common strategy to improve the properties of oligonucleotides, particularly for therapeutics and nanotechnology. Existing synthetic methods essentially rely on phosphoramidite chemistry or the polymerization of nucleosid
Protein synthesis represents the major route of disposal of amino acids. Amino acids are activated by binding to specific molecules of transfer RNA and assembled by ribosomes into a sequence that has been specified by messenger RNA, which in turn has been transcribed from the DNA template. Peptid...
USE OF TEMPLATE SWITCHING FOR DNA SY NT H ESIS 发明人:LAMBOWITZ, Alan, M.,MOHR, Sabine,WHITE, Travis, B.,KUERSTEN, Scott 申请号:EP 1274 994 2.4 申请日:20120223 公开号:EP 26784 30A1 公开日:2014 0101 摘要:A method of preparing a DNA copy of a target polynucleotide using template ...
A DNA-Unwinding Protein Isolated from Escherichia coli: Its Interaction with DNA and with DNA Polymerases A DNA-unwinding protein has been purified to homogeneity from E. coli. This protein has a molecular weight of about 22,000, as judged by its electrophoreti... N Sigal,H Delius,T Kornbe...
We herein demonstrate an innovative example of TGS using DNA nano-templates that promote Huisgen cycloaddition from an array of azide and alkyne fragments. A G-quadruplex and a control duplex DNA nano-template have been prepared by assembling the DNA structures on gold-coated magnetic nanoparticles....