Human and murine ICAM-1-targeting CAR T cells displayed strong lysis of ICAM-1-expressing glioma and endothelial cells in vitro. Intratumoral application of CAR T cells resulted in a survival benefit in both syngeneic and xenograft glioma mouse models. Ex vivo analysis of the tumor ...
As the science evolves, we have worked to enhance our portfolio with additional mouse models of cancer and will continue to expand into additional models that allow for this simulationin vitroandin vivo,includinghumanized models, genetically altered models, andcytokine response, T-cell...
X Delete Research Models Syngeneic Models >> Request a Quote or Information Our Experts will contact you providing a quote, information and estimated timeframe to your project needs. The syngeneic model (also known as allograft mouse tumor systems) involves inoculation of tumor cell lines (derived...
Cav-1 KO 4T1 cells and a syngeneic mouse model of advanced BC were used to study Cav-1 function in BC progression and metastasis in a physiologically relevant system. The effects of Cav-1 deficiency on cellular behaviors, including migration, wound closure, MMP secretion, and gene expression,...
Syngeneic mouse tumor models are generally non-permissive for adenoviral replication, whereas human tumor xenograft models exhibit attenuated immune responses to the vector. The cotton rat (Sigmodon hispidus) is susceptible to human adenovirus infection, permissive for viral replication and exhibits similar...
(C) AF647-labeled CATs and alum were incubated for 30 min in TBS, then resuspended in PBS + 10% mouse serum at t = 0 and replaced at the indicated time points. Fluorescence spectroscopy was used to measure the percentage of protein bound to alum over time; mean±SD (n = 3). (D...
Since these syngeneic mouse models have relatively low T-lymphocyte infiltration, they may represent T-cell excluded human tumors. In addition to neoantigens from somatic mutations, neoantigens from endogenous viral proteins can also act as tumor associated antigens and elicit CD8 T cell im- m...
B7-H3 nanobody CAR-T cells were generated from mouse splenic T cells. In vitro tumor killing assays were performed by xCELLigence. sTREM2 GEMys were generated from bone marrow progenitor cells using lentiviral transduction. For in vivo studies, mice underwent orthotopic implantation of tumors and ...
Methods: To assess the immune impacts of our huHER2-targeted combination strategy, we generated a syngeneic mouse model of huHER2 breast cancer, using a truncated form of huHER2, HER2T. Following validation of this model, we next treated tumour-bearing with our immuno...
Only one or two identical T cell clonotypes were detected in each animal, with mouse 4 being a clear exception. In this animal, six clonotypes were detected in more than one tumor and two of these were detected in all three lesions. Prompted by this ®nding we also ana- lyzed PBL,...