AcetaminnoopphheennaannddCCooddeeininee对乙酰氨基酚和可待因 HPLC Method Column: Part Number: Mobile phase: Flow rate: Injection volume: Detection: Symmetry® C8, 3.9 x 150mm, 5 µm WAT046970 10mM docusate sodium in methanol/water/THF/phosphoric acid 60:36:4:1 0.75mL/min...
Analytical HPLC was performed on Jasco 2000 series HPLC systems using a Phenomenex ‘‘Kinetex’’ 5 μm particle size, 100 Å pore size, C18 column of dimensions 100 × 4.6 mm. Chromatograms were monitored at 220 and 280 nm. Gradients were 20 to 80% or 40 to 100% acet...
DNH and DPC were optically resolved by chiral HPLC (Supplementary Figures 4, 5). CD spectra of (P,P)-DNH and (P,P)-DPC, as well as (P)-HH, were recorded in dichloromethane at 25 °C (Fig. 3a); for the corresponding UV–Vis spectra and gabs factor profiles, see Supplementary ...
c.InitialColumnEfficiencyDetermination iii.columnuse a.GuardColumns b.SamplePreparation c.pHRange d.Solvents e.Pressure f.Temperature iV.scalinGup/downisocraticmethods V.troubleshootinG Vi.columncleaninG,reGeneratinGandstoraGe a.CleaningandRegenerating b.Storage Vii.connectinGthecolumntothehplc a.Column...
This influence is attributed to the disruption of the analyte solvation鈥揹esolvation equilibria in the mobile phase, also known as "chaotropic effect". With an increase of counteranion concentration analyte retention increases with concomitant decrease in the tailing factor. Different inorganic ...
contain a protein known as bacterioferritin-associated ferredoxin27. These ferredoxins bind iron–sulfur clusters, are upregulated 200-fold in response to a low iron environment and are required to liberate any iron from within the bacterioferritin compartments27. A similar undiscovered factor may ...
The presence of carbohydrate residues was found to be a critical factor in the separation of enantiomers, affecting both selectivity and peak resolution. CSPs with intermediate products of hydrolysis demonstrated the best resolution of β-blocker enantiomers. Under optimum conditions, the separation of ...
Kuhn‟s anisotropy factor in the ground state was defined as gCD = 2×(L–R)/(L+R), where L and R, respectively, denoted the molar absorptivity for left and right circularly polarized light. The magnitude of circular polarization in the excited states was defined ...