SV40起源 sv40 origin SV40起源
Effect of human cytomegalovirus on replication of SV40 origin and the expression of T antigen. Virology 177: 824–828 - Jeor - 1990 () Citation Context ... SV40-infected cells. In this respect, EBV could behave like other herpesviruses, namely, herpes simplex virus and human cytomegalovirus, ...
Assembly of transfected DNA into chromatin: structural changes in the origin-promoter-enhancer region upon replication. Chimeric SV40 DNA containing only the early region, or plasmid DNA harboring the origin-promoter-enhancer region of SV40, when introduced into CV-1 or Cos-... Cereghini,Yaniv -...
We report here that expression of mouse p53 results in a substantial and selective inhibition of SV40 origin-dependent DNA replication. In addition to any function in the G0-G1 transition, the data presented suggest that p53 may affect directly the initiation or maintenance of replicative DNA ...
Origin of SV40 DNA ReplicationNo Abstract available for this article.doi:10.1038/236329b0NoneNature Publishing Group UKNature
故特称为COS(CV-l,origin, SV40)细胞系(请注意,不要把COS细胞系同cos粘性末端位点混淆!)。由于在COS细胞系的基因组中,整合有SV40早期区段DNA,故能够组成地表达T抗原。 应用这种复制功能缺陷的SV40病毒突变株转化人的成纤维细胞。同样也获得了一种其功能与COS细胞类似的新型的细胞系HFS(human fibroblasts,SV40)...
COS-7细胞源自CV-1细胞,经带有编码野生型T抗原的起始失活突变的SV40转染得到。COS-7细胞含有一个来自SV40基因组全部早期区域的组成型拷贝。细胞别称: Cos-7; COS7; Cos7; CV-1 in Origin Simian-7。 提示:细胞内黑色颗粒偏多,传代会有部分到培养基内,注意及时清除细胞外黑色碎片。我们推荐使用尚恩生物非洲绿...
SV40 vectors carrying minimal sequence of viral origin with exchangeable capsids. Polyomaviral vectors are generated by transfecting 293T cells with three sets of DNAs: DNA for the expression of simian virus 40 (SV40) T antigen; DNA for ... A Nakanishi,B Chapellier,N Maekawa,... - 《Vi...
rep_origin 270..347 /label="SV40_origin" misc_feature 332..351 /label="SV40pro_F_primer" misc_feature 489..621 /label="chim_int" promoter 666..684 /label="T7_promoter" CDS 694..1629 /label="ORF frame 1" terminator complement(1657..1776) ...
3 2.强调“一些终止序列”的原因：•（1）DNA有线状和环状、平齐末端、粘性末端多种形式，所以终点序列形式多样性。•（2）“终止序列”用于复制中。“终止子”用于转录中。4 4.“复制起点”Origin •即复制原点。•（1）代号：“ori”,“oriC”,“O”。•（2）原核生物“ori”有五点特征：（见...